論文 - 詳細
| RRC ID | 19004 |
|---|---|
| 著者 | Lee CM, Sieo CC, Cheah YK, Abdullah N, Ho YW. |
| タイトル | Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting. |
| ジャーナル | J Sci Food Agric |
| Abstract |
BACKGROUND:Four repetitive element sequence-based polymerase chain reaction (rep-PCR) methods, namely repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), polytrinucleotide (GTG)₅ -PCR and BOX-PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep-PCR methods and their combination (composite rep-PCR) in the molecular typing of Lactobacillus strains based on a discriminatory index (D). RESULTS:Species-specific and strain-specific profiles were observed from rep-PCR. From the numerical analysis of composite rep-PCR, BOX-PCR, (GTG)₅ -PCR, REP-PCR and ERIC-PCR, D values of 0.9118, 0.9044, 0.8897, 0.8750 and 0.8529 respectively were obtained. Composite rep-PCR analysis was the most discriminative method, with eight Lactobacillus strains, namely L. brevis ATCC 14869(T) , L. reuteri C 10, L. reuteri ATCC 23272(T) , L. gallinarum ATCC 33199(T) , L. salivarius ATCC 11741(T) , L. salivarius I 24, L. panis JCM 11053(T) and L. panis C 17, being differentiated at the strain level. CONCLUSION:Composite rep-PCR analysis is potentially a useful fingerprinting method to discriminate probiotic Lactobacillus strains isolated from the gastrointestinal tract of chickens. |
| 巻・号 | 92(3) |
| ページ | 660-6 |
| 公開日 | 2012-2-1 |
| DOI | 10.1002/jsfa.4627 |
| PMID | 21919004 |
| MeSH | Animals Chickens / microbiology DNA Fingerprinting / veterinary DNA, Bacterial / metabolism* Diet / veterinary* Gastrointestinal Tract / microbiology Intestinal Mucosa / microbiology Inverted Repeat Sequences Lactobacillus / classification* Lactobacillus / genetics Lactobacillus / isolation & purification Lactobacillus / metabolism Malaysia Molecular Typing / methods Molecular Typing / veterinary Phylogeny Polymerase Chain Reaction / veterinary Probiotics / classification* Probiotics / isolation & purification Probiotics / metabolism Repetitive Sequences, Nucleic Acid Species Specificity |
| IF | 2.614 |
| 引用数 | 8 |
| WOS 分野 | AGRICULTURE, MULTIDISCIPLINARY FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED |
| リソース情報 | |
| 一般微生物 | JCM 11053 |