論文 - 詳細
| RRC ID | 19022 |
|---|---|
| 著者 | Yang G, Benson RF, Ratcliff RM, Brown EW, Steigerwalt AG, Thacker WL, Daneshvar MI, Morey RE, Saito A, Fields BS. |
| タイトル | Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel Legionella species was identified based on analysis of 16S rRNA and mip (macrophage infectivity potentiator) gene sequences, cellular fatty acids, isoprenoid quinones, biochemical reactions, antigens and quantitative DNA-DNA hybridization. Strain CDC-1796-JAP-E(T) was isolated from well water at the Nagasaki Municipal Medical Center, Japan. Two strains, CDC-3041-AUS-E and CDC-3558-AUS-E, were isolated from water samples during an outbreak of legionellosis in South Australia. Strain CDC-5427-OH-H was isolated from a 66-year-old female patient diagnosed with Legionnaires' disease in the US. Cells from these four strains were gram-negative, non-fluorescent, rod-shaped, and positive for alkaline phosphatase, esterase, leucine arylamidase, catalase, gelatinase, β-lactamase and tyrosine browning assay. Phylogenetic analysis of 16S rRNA and mip genes revealed that the four strains formed a distinct cluster within the genus Legionella. The bacteria contained branched-chain fatty acids and quinones that are typical of members of the genus Legionella. Slide agglutination tests demonstrated no cross-reaction with 52 previously described members of the Legionellaceae. DNA-DNA hybridization studies indicated that DNAs from the four strains were highly related (78-84 %) but they showed 29 % relatedness to Legionella oakridgensis ATCC 33761(T) and less than 10 % to strains of other Legionella species tested. These characterizations suggest that the isolates represent a novel species, for which the name Legionella nagasakiensis sp. nov. is proposed; the type strain is CDC-1796-JAP-E(T) ( = ATCC BAA-1557(T) = JCM 15315(T)). |
| 巻・号 | 62(Pt 2) |
| ページ | 284-288 |
| 公開日 | 2012-2-1 |
| DOI | 10.1099/ijs.0.027193-0 |
| PMID | 21398499 |
| MeSH | Aged Bacterial Proteins / genetics Bacterial Typing Techniques DNA, Bacterial / analysis DNA, Ribosomal / analysis Fatty Acids / analysis Female Fresh Water / microbiology* Genes, rRNA Humans Japan / epidemiology Legionella / classification* Legionella / genetics Legionella / isolation & purification* Legionella / physiology Legionellosis / microbiology* Molecular Sequence Data Nucleic Acid Hybridization Peptidylprolyl Isomerase / genetics Phylogeny Pneumonia, Bacterial / microbiology* Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA South Australia / epidemiology Species Specificity United States / epidemiology Water Supply* |
| IF | 2.415 |
| 引用数 | 8 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 15315 |