| RRC ID |
1905
|
| Author |
Omoteyama K, Ikeda H, Imaki J, Sakai M.
|
| Title |
Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The Maf family of transcription factors is expressed during development of various organs and tissues, and is involved in a variety of developmental and cellular differentiation processes. We previously found that c-maf and mafB are strongly expressed in hypertrophic chondrocytes during cartilage development. Connective tissue growth factor (CTGF) is also expressed in hypertrophic chondrocytes. Adenovirus mediated introduction of c-maf gene into the mouse fibroblast cell line C3H10T1/2 strongly induced CTGF expression. CTGF can be induced by TGF-beta via the SMAD pathway; however, the c-Maf could not induce TGF-beta, nor could TGF-beta induce the c-Maf, suggesting that activation of CTGF by Maf is TGF-beta independent. Reporter transfection analysis using C3H10T1/2 cells shows that c-Maf stimulates a CTGF reporter gene. Lc-Maf, a splice variant of c-Maf containing an extra 10 amino acids in the carboxyl terminus, was a stronger inducer of the CTGF reporter gene than c-Maf. Chromatin immunoprecipitation analysis showed that c-Maf binds to the promoter region of the CTGF gene, indicating that Maf directly activates the CTGF gene. Taken together, these data indicate that the CTGF gene is a target of c-Maf and Lc-Maf in cartilage development.
|
| Volume |
339(4)
|
| Pages |
1089-97
|
| Published |
2006-1-27
|
| DOI |
10.1016/j.bbrc.2005.11.119
|
| PII |
S0006-291X(05)02647-1
|
| PMID |
16343439
|
| MeSH |
Animals
Cell Differentiation
Chondrocytes / cytology*
Chondrocytes / metabolism*
Connective Tissue Growth Factor
Fibroblasts / cytology*
Fibroblasts / metabolism*
Gene Expression Regulation / physiology
Immediate-Early Proteins / metabolism*
Intercellular Signaling Peptides and Proteins / metabolism*
Mice
Mice, Inbred C3H
Promoter Regions, Genetic
Proto-Oncogene Proteins c-maf / metabolism*
Transcription Factors / metabolism
Transcriptional Activation / physiology
|
| IF |
2.985
|
| Times Cited |
11
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
AxCALNLrmaf2 (RDB02410)
AxCANCre (RDB01748). |
