RRC ID 19060
著者 Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC.
タイトル Azospirillum formosense sp. nov., a diazotroph from agricultural soil.
ジャーナル Int J Syst Evol Microbiol
Abstract A gram-negative, spiral or rod-shaped, non-spore-forming diazotrophic bacterium, designated CC-Nfb-7(T), was isolated from agricultural soil in Yunlin County, Taiwan. 16S rRNA gene sequence analysis showed that strain CC-Nfb-7(T) was most closely related to Azospirillum brasilense DSM 1690(T) (97.4 % 16S rRNA gene sequence similarity), Azospirillum rugosum IMMIB AFH-6(T) (96.8 %) and Azospirillum oryzae JCM 21588(T) (96.6 %); <96.5 % 16S rRNA gene sequence similarity was found with all other members of the genus Azospirillum. DNA-DNA relatedness between strain CC-Nfb-7(T) and A. brasilense DSM 1690(T), A. rugosum DSM 19657(T) and A. oryzae JCM 21588(T) was 38.9, 30.1 and 31.8 %, respectively. The respiratory quinone was ubiquinone Q-10. The major fatty acids were summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c), summed feature 3 (consisting of C(16 : 1)ω7c and/or C(16 : 1)ω6c), summed feature 2 (consisting of C(14 : 0) 3-OH and/or iso-C(16 : 1) I), C(16 : 0), C(18 : 0) 2-OH and C(16 : 0) 3-OH. The polar lipids consisted mainly of phosphatidylglycerol, phosphatidylcholine and one unidentified phospholipid. Furthermore, moderate amounts of phosphatidylethanolamine, phosphatidyldimethylethanolamine and one unidentified aminophospholipid were also detected. Strain CC-Nfb-7(T) could be distinguished from members of phylogenetically related species by differences in phenotypic properties. On the basis of morphological, chemotaxonomic and phylogenetic data, strain CC-Nfb-7(T) represents a novel species within the genus Azospirillum, for which we propose the name Azospirillum formosense sp. nov. The type strain is CC-Nfb-7(T) ( = BCRC 80273(T) = JCM 17639(T) = DSM 24137(T)).
巻・号 62(Pt 5)
ページ 1185-1190
公開日 2012-5-1
DOI 10.1099/ijs.0.030585-0
PMID 21742820
MeSH Azospirillum brasilense / classification* Azospirillum brasilense / genetics Azospirillum brasilense / isolation & purification* Azospirillum brasilense / metabolism Bacterial Typing Techniques Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Molecular Sequence Data Nitrogen Fixation Nucleic Acid Hybridization Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Taiwan
IF 2.415
引用数 21
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17639 JCM 21588