論文 - 詳細
| RRC ID | 19072 |
|---|---|
| 著者 | Phillips G, Swairjo MA, Gaston KW, Bailly M, Limbach PA, Iwata-Reuyl D, de Crécy-Lagard V. |
| タイトル | Diversity of archaeosine synthesis in crenarchaeota. |
| ジャーナル | ACS Chem Biol |
| Abstract |
Archaeosine (G(+)) is found at position 15 of many archaeal tRNAs. In Euryarchaeota, the G(+) precursor, 7-cyano-7-deazaguanine (preQ(0)), is inserted into tRNA by tRNA-guanine transglycosylase (arcTGT) before conversion into G(+) by ARChaeosine Synthase (ArcS). However, many Crenarchaeota known to harbor G(+) lack ArcS homologues. Using comparative genomics approaches, two families that could functionally replace ArcS in these organisms were identified: (1) GAT-QueC, a two-domain family with an N-terminal glutamine amidotransferase class-II domain fused to a domain homologous to QueC, the enzyme that produces preQ(0) and (2) QueF-like, a family homologous to the bacterial enzyme catalyzing the reduction of preQ(0) to 7-aminomethyl-7-deazaguanine. Here we show that these two protein families are able to catalyze the formation of G(+) in a heterologous system. Structure and sequence comparisons of crenarchaeal and euryarchaeal arcTGTs suggest the crenarchaeal enzymes have broader substrate specificity. These results led to a new model for the synthesis and salvage of G(+) in Crenarchaeota. |
| 巻・号 | 7(2) |
| ページ | 300-5 |
| 公開日 | 2012-2-17 |
| DOI | 10.1021/cb200361w |
| PMID | 22032275 |
| PMC | PMC3289047 |
| MeSH | Amino Acid Sequence Archaeal Proteins / chemistry Archaeal Proteins / genetics Archaeal Proteins / metabolism* Crenarchaeota / chemistry Crenarchaeota / enzymology* Crenarchaeota / genetics Crenarchaeota / metabolism Genomics Guanosine / analogs & derivatives* Guanosine / chemistry Guanosine / metabolism Molecular Sequence Data Phylogeny Sequence Alignment Substrate Specificity |
| IF | 4.434 |
| 引用数 | 25 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 11548 |