RRC ID 19080
Author Grégoire P, Fardeau ML, Guasco S, Lagière J, Cambar J, Michotey V, Bonin P, Ollivier B.
Title Desulfosoma profundi sp. nov., a thermophilic sulfate-reducing bacterium isolated from a deep terrestrial geothermal spring in France.
Journal Antonie Van Leeuwenhoek
Abstract A novel strictly anaerobic bacterium designated SPDX02-08(T) was isolated from a deep terrestrial geothermal spring located in southwest France. Cells (1-2 × 2-6 μm) were non-motile, non sporulating and stained Gram negative. Strain SPDX02-08(T) grew at a temperature between 40 and 60°C (optimum 55°C), pH between 6.3 and 7.3 (optimum 7.2) and a NaCl concentration between 0 and 5 g/l (optimum 2 g/l). Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental sulfur, nitrate, nitrite, Fe (III) or fumarate. In the presence of sulfate, strain SPDX02-08(T) completely oxidized pyruvate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexadecanoate. Stoichiometric measurements revealed a complete oxidation of part of lactate (0.125 mol of acetate produced per mole lactate oxidized). Strain SPDX02-08(T) required yeast extract to oxidize formate and H(2) but did not grow autotrophically on H(2). Among the substrates tested, only pyruvate was fermented. The G+C content of the genomic DNA was 57.6 mol%. Major cellular fatty acids of strain SPDX02-08(T) were iso-C(15:0), C(15:0), and C(16:0). Phylogenetic analysis of the 16S small-subunit (SSU) ribosomal RNA gene sequence indicated that strain SPDX02-08(T) belongs to the genus Desulfosoma, family Syntrophobacteraceae, having Desulfosoma caldarium as its closest phylogenetic relative (97.6% similarity). The mean DNA/DNA reassociation value between strain SPDX02-08(T) and Desulfosoma caldarium was 16.9 ± 2.7%. Based on the polyphasic differences, strain SPDX02-08(T) is proposed to be assigned as a new species of the genus Desulfosoma, Desulfosoma profundi sp. nov. (DSM 22937(T) = JCM 16410(T)). GenBank accession number for the 16S rRNA gene sequence of strain SPDX02-08(T) is HM056226.
Volume 101(3)
Pages 595-602
Published 2012-3
DOI 10.1007/s10482-011-9675-x
PMID 22120904
MeSH Anaerobiosis DNA, Bacterial / genetics DNA, Ribosomal / genetics Deltaproteobacteria / classification Deltaproteobacteria / genetics Deltaproteobacteria / isolation & purification* Deltaproteobacteria / metabolism Deltaproteobacteria / ultrastructure Fatty Acids / metabolism France Hot Springs / microbiology* Molecular Sequence Data Oxidation-Reduction Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Alignment Sequence Homology, Nucleic Acid Species Specificity Sulfates / metabolism* Water Microbiology*
IF 1.588
Times Cited 3
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 16410