RRC ID 19097
著者 Thong-On A, Suzuki K, Noda S, Inoue J, Kajiwara S, Ohkuma M.
タイトル Isolation and characterization of anaerobic bacteria for symbiotic recycling of uric acid nitrogen in the gut of various termites.
ジャーナル Microbes Environ
Abstract Recycling of the nitrogenous waste uric acid (UA) of wood-feeding termites by their gut bacteria is one of the significant aspects of symbiosis for the conservation of nitrogen sources. Diverse anaerobic UA-degrading bacteria comprising 16 species were isolated from the gut of eight termite species, and were assigned to Clostridia, Enterobacteriaceae, and low G+C Gram-positive cocci. UA-degrading Clostridia had never been isolated from termite guts. UA-degrading ability was sporadically distributed among phylogenetically various culturable anaerobic bacteria from termite guts. A strain of Clostridium sp., which was commonly isolated from three termite species and represented a probable new species in cluster XIVa of clostridia, utilized UA as a nitrogen source but not as a sole carbon and energy source. This feature is in clear contrast to that of well-studied purinolytic clostridia or previously isolated UA degraders from termite guts, which also utilize UA as a sole carbon and energy source. Ammonia is the major nitrogenous product of UA degradation. Various purines stimulated the growth of this strain when added to an otherwise growth-limiting, nitrogen poor medium. The bacterial species involved the recycling of UA nitrogen in the gut microbial community of termites are more diverse in terms of both taxonomy and nutritional physiology than previously recognized.
巻・号 27(2)
ページ 186-92
公開日 2012-1-1
DOI 10.1264/jsme2.me11325
PII DN/JST.JSTAGE/jsme2/ME11325
PMID 22791052
PMC PMC4036019
MeSH Ammonia / metabolism Animals Bacteria, Anaerobic / classification Bacteria, Anaerobic / isolation & purification* Bacteria, Anaerobic / metabolism Bacteria, Anaerobic / physiology* Biodiversity* Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Gastrointestinal Tract / microbiology Isoptera / microbiology* Molecular Sequence Data Nitrogen / metabolism* Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Symbiosis* Uric Acid / metabolism*
IF 2.575
引用数 30
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 10513 JCM 10514 JCM 10515 JCM 10519 JCM 10522 JCM 10521 JCM 10523 JCM 10537 JCM 1297 JCM 1415 JCM 17987 JCM 17988 JCM 17989 JCM 17990 JCM 17991 JCM 17992 JCM 17993 JCM 17994