論文 - 詳細
| RRC ID | 19098 |
|---|---|
| 著者 | Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, Tanabe S. |
| タイトル | Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258(T) on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258(T) suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258(T) was growth phase- and culture temperature-dependent. Treatment of ATCC 19258(T) with Mg(2+) decreased the dlt mRNA expression and D-Ala content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with L-alanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258(T) and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala. |
| 巻・号 | 76(2) |
| ページ | 283-8 |
| 公開日 | 2012-1-1 |
| DOI | 10.1271/bbb.110646 |
| PII | JST.JSTAGE/bbb/110646 |
| PMID | 22313760 |
| MeSH | Alanine / pharmacology* Caco-2 Cells Cell Culture Techniques Cell Wall / chemistry Epithelial Cells / physiology Humans Intestinal Mucosa / cytology Intestinal Mucosa / metabolism Intestinal Mucosa / physiology* Permeability Streptococcus thermophilus / chemistry* Teichoic Acids / chemistry Teichoic Acids / pharmacology* Tumor Necrosis Factor-alpha / physiology |
| IF | 1.516 |
| 引用数 | 2 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1222 |