RRC ID 19098
Author Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, Tanabe S.
Title Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells.
Journal Biosci. Biotechnol. Biochem.
Abstract D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258(T) on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258(T) suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258(T) was growth phase- and culture temperature-dependent. Treatment of ATCC 19258(T) with Mg(2+) decreased the dlt mRNA expression and D-Ala content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with L-alanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258(T) and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.
Volume 76(2)
Pages 283-8
Published 2012
DOI 10.1271/bbb.110646
PII JST.JSTAGE/bbb/110646
PMID 22313760
MeSH Alanine / pharmacology* Caco-2 Cells Cell Culture Techniques Cell Wall / chemistry Epithelial Cells / physiology Humans Intestinal Mucosa / cytology Intestinal Mucosa / metabolism Intestinal Mucosa / physiology* Permeability Streptococcus thermophilus / chemistry* Teichoic Acids / chemistry Teichoic Acids / pharmacology* Tumor Necrosis Factor-alpha / physiology
IF 1.255
Times Cited 1
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 1222