| RRC ID |
19098
|
| 著者 |
Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, Tanabe S.
|
| タイトル |
Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258(T) on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258(T) suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258(T) was growth phase- and culture temperature-dependent. Treatment of ATCC 19258(T) with Mg(2+) decreased the dlt mRNA expression and D-Ala content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with L-alanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258(T) and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.
|
| 巻・号 |
76(2)
|
| ページ |
283-8
|
| 公開日 |
2012-1-1
|
| DOI |
10.1271/bbb.110646
|
| PII |
JST.JSTAGE/bbb/110646
|
| PMID |
22313760
|
| MeSH |
Alanine / pharmacology*
Caco-2 Cells
Cell Culture Techniques
Cell Wall / chemistry
Epithelial Cells / physiology
Humans
Intestinal Mucosa / cytology
Intestinal Mucosa / metabolism
Intestinal Mucosa / physiology*
Permeability
Streptococcus thermophilus / chemistry*
Teichoic Acids / chemistry
Teichoic Acids / pharmacology*
Tumor Necrosis Factor-alpha / physiology
|
| IF |
1.516
|
| 引用数 |
2
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1222 |
