論文 - 詳細
| RRC ID | 19113 |
|---|---|
| 著者 | Lü Z, Lu Y. |
| タイトル | Methanocella conradii sp. nov., a thermophilic, obligate hydrogenotrophic methanogen, isolated from Chinese rice field soil. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Methanocellales contributes significantly to anthropogenic methane emissions that cause global warming, but few pure cultures for Methanocellales are available to permit subsequent laboratory studies (physiology, biochemistry, etc.). METHODOLOGY/PRINCIPAL FINDINGS:By combining anaerobic culture and molecular techniques, a novel thermophilic methanogen, strain HZ254(T) was isolated from a Chinese rice field soil located in Hangzhou, China. The phylogenetic analyses of both the 16S rRNA gene and mcrA gene (encoding the α subunit of methyl-coenzyme M reductase) confirmed its affiliation with Methanocellales, and Methanocella paludicola SANAE(T) was the most closely related species. Cells were non-motile rods, albeit with a flagellum, 1.4-2.8 µm long and by 0.2-0.3 µm in width. They grew at 37-60 °C (optimally at 55 °C) and salinity of 0-5 g NaCl l(-1) (optimally at 0-1 g NaCl l(-1)). The pH range for growth was 6.4-7.2 (optimum 6.8). Under the optimum growth condition, the doubling time was 6.5-7.8 h, which is the shortest ever observed in Methanocellales. Strain HZ254(T) utilized H(2)/CO(2) but not formate for growth and methane production. The DNA G+C content of this organism was 52.7 mol%. The sequence identities of 16S rRNA gene and mcrA gene between strain HZ254(T) and SANAE(T) were 95.0 and 87.5% respectively, and the genome based Average Nucleotide Identity value between them was 74.8%. These two strains differed in phenotypic features with regard to substrate utilization, possession of a flagellum, doubling time (under optimal conditions), NaCl and temperature ranges. Taking account of the phenotypic and phylogenetic characteristics, we propose strain HZ254(T) as a representative of a novel species, Methanocella conradii sp. nov. The type strain is HZ254(T) ( = CGMCC 1.5162(T) = JCM 17849(T) = DSM 24694(T)). CONCLUSIONS/SIGNIFICANCE:Strain HZ254(T) could potentially serve as an excellent laboratory model for studying Methanocellales due to its fast growth and consistent cultivability. |
| 巻・号 | 7(4) |
| ページ | e35279 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0035279 |
| PII | PONE-D-12-00821 |
| PMID | 22530002 |
| PMC | PMC3328440 |
| MeSH | Base Composition DNA Restriction Enzymes / genetics Methanomicrobiaceae / classification Methanomicrobiaceae / genetics* Methanomicrobiaceae / growth & development Methanomicrobiaceae / ultrastructure Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S Soil Microbiology* |
| IF | 2.74 |
| 引用数 | 32 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 17849 |