RRC ID 19119
Author Martínez MG, Prado Acosta M, Candurra NA, Ruzal SM.
Title S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection.
Journal Biochem. Biophys. Res. Commun.
Abstract It has been previously described that S-layer binds to the C-type lectin DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN, CD209). It was also shown that DC-SIGN is a cell-surface adhesion factor that enhances viral entry of several virus families. Among those, Junin virus (JUNV) entry is enhanced in cells expressing DC-SIGN and for that reason surface-layer protein (S-layer) of Lactobacillus acidophilus ATCC 4365 was evaluated as a possible JUNV inhibitor. Experiments using 3T3 cells stably expressing DC-SIGN, showed an almost complete inhibition of JUNV infection when they were treated with S-layer in a similar extend as the inhibition shown by mannan. However no inhibition effect was observed in 3T3 wild type cells or in 3T3 cells expressing liver/lymph node-specific ICAM-3 grabbing nonintegrin (L-SIGN or DC-SIGNR or CD209L). Treatments with S-layer during different times in the infection demonstrated that inhibition was only observed when S-layer was presented in early stages of the viral infection. This inhibition does not involve the classic recognition of mannose by this C-type lectin as the S-layer showed no evidence to be glycosylated. In fact, the highly basic nature of the S-layer (pI>9.5) seems to be involved in electrostatic interactions between DC-SIGN and S-layer, since high pH abolished the inhibitory effect on infection cause by the S-layer. In silico analysis predicts a Ca(2+)-dependant carbohydrate recognition domain in the SlpA protein. This novel characteristic of the S-layer, a GRAS status protein, contribute to the pathogen exclusion reported for this probiotic strain and may be applied as an antiviral agent to inhibit several kinds of viruses.
Volume 422(4)
Pages 590-5
Published 2012-6-15
DOI 10.1016/j.bbrc.2012.05.031
PII S0006-291X(12)00895-9
PMID 22595457
MeSH 3T3 Cells Amino Acid Sequence Animals Antiviral Agents / chemistry Antiviral Agents / isolation & purification Antiviral Agents / pharmacology* Arenaviridae Infections Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism Cercopithecus aethiops Consensus Sequence Junin virus / drug effects* Lactobacillus acidophilus* Lectins, C-Type / genetics Lectins, C-Type / metabolism Membrane Glycoproteins / chemistry Membrane Glycoproteins / isolation & purification Membrane Glycoproteins / pharmacology* Mice Molecular Sequence Data Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Vero Cells Virus Internalization / drug effects*
IF 2.559
Times Cited 19
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 5818