論文 - 詳細
| RRC ID | 19119 |
|---|---|
| 著者 | Martínez MG, Prado Acosta M, Candurra NA, Ruzal SM. |
| タイトル | S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
It has been previously described that S-layer binds to the C-type lectin DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN, CD209). It was also shown that DC-SIGN is a cell-surface adhesion factor that enhances viral entry of several virus families. Among those, Junin virus (JUNV) entry is enhanced in cells expressing DC-SIGN and for that reason surface-layer protein (S-layer) of Lactobacillus acidophilus ATCC 4365 was evaluated as a possible JUNV inhibitor. Experiments using 3T3 cells stably expressing DC-SIGN, showed an almost complete inhibition of JUNV infection when they were treated with S-layer in a similar extend as the inhibition shown by mannan. However no inhibition effect was observed in 3T3 wild type cells or in 3T3 cells expressing liver/lymph node-specific ICAM-3 grabbing nonintegrin (L-SIGN or DC-SIGNR or CD209L). Treatments with S-layer during different times in the infection demonstrated that inhibition was only observed when S-layer was presented in early stages of the viral infection. This inhibition does not involve the classic recognition of mannose by this C-type lectin as the S-layer showed no evidence to be glycosylated. In fact, the highly basic nature of the S-layer (pI>9.5) seems to be involved in electrostatic interactions between DC-SIGN and S-layer, since high pH abolished the inhibitory effect on infection cause by the S-layer. In silico analysis predicts a Ca(2+)-dependant carbohydrate recognition domain in the SlpA protein. This novel characteristic of the S-layer, a GRAS status protein, contribute to the pathogen exclusion reported for this probiotic strain and may be applied as an antiviral agent to inhibit several kinds of viruses. |
| 巻・号 | 422(4) |
| ページ | 590-5 |
| 公開日 | 2012-6-15 |
| DOI | 10.1016/j.bbrc.2012.05.031 |
| PII | S0006-291X(12)00895-9 |
| PMID | 22595457 |
| PMC | PMC7124250 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Antiviral Agents / chemistry Antiviral Agents / isolation & purification Antiviral Agents / pharmacology* Arenaviridae Infections Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism Chlorocebus aethiops Consensus Sequence Junin virus / drug effects* Lactobacillus acidophilus* Lectins, C-Type / genetics Lectins, C-Type / metabolism Membrane Glycoproteins / chemistry Membrane Glycoproteins / isolation & purification Membrane Glycoproteins / pharmacology* Mice Molecular Sequence Data Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Vero Cells Virus Internalization / drug effects* |
| IF | 2.985 |
| 引用数 | 37 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 5818 |