| RRC ID |
19119
|
| 著者 |
Martínez MG, Prado Acosta M, Candurra NA, Ruzal SM.
|
| タイトル |
S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
It has been previously described that S-layer binds to the C-type lectin DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN, CD209). It was also shown that DC-SIGN is a cell-surface adhesion factor that enhances viral entry of several virus families. Among those, Junin virus (JUNV) entry is enhanced in cells expressing DC-SIGN and for that reason surface-layer protein (S-layer) of Lactobacillus acidophilus ATCC 4365 was evaluated as a possible JUNV inhibitor. Experiments using 3T3 cells stably expressing DC-SIGN, showed an almost complete inhibition of JUNV infection when they were treated with S-layer in a similar extend as the inhibition shown by mannan. However no inhibition effect was observed in 3T3 wild type cells or in 3T3 cells expressing liver/lymph node-specific ICAM-3 grabbing nonintegrin (L-SIGN or DC-SIGNR or CD209L). Treatments with S-layer during different times in the infection demonstrated that inhibition was only observed when S-layer was presented in early stages of the viral infection. This inhibition does not involve the classic recognition of mannose by this C-type lectin as the S-layer showed no evidence to be glycosylated. In fact, the highly basic nature of the S-layer (pI>9.5) seems to be involved in electrostatic interactions between DC-SIGN and S-layer, since high pH abolished the inhibitory effect on infection cause by the S-layer. In silico analysis predicts a Ca(2+)-dependant carbohydrate recognition domain in the SlpA protein. This novel characteristic of the S-layer, a GRAS status protein, contribute to the pathogen exclusion reported for this probiotic strain and may be applied as an antiviral agent to inhibit several kinds of viruses.
|
| 巻・号 |
422(4)
|
| ページ |
590-5
|
| 公開日 |
2012-6-15
|
| DOI |
10.1016/j.bbrc.2012.05.031
|
| PII |
S0006-291X(12)00895-9
|
| PMID |
22595457
|
| PMC |
PMC7124250
|
| MeSH |
3T3 Cells
Amino Acid Sequence
Animals
Antiviral Agents / chemistry
Antiviral Agents / isolation & purification
Antiviral Agents / pharmacology*
Arenaviridae Infections
Bacterial Proteins / isolation & purification
Bacterial Proteins / pharmacology*
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / metabolism
Chlorocebus aethiops
Consensus Sequence
Junin virus / drug effects*
Lactobacillus acidophilus*
Lectins, C-Type / genetics
Lectins, C-Type / metabolism
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / isolation & purification
Membrane Glycoproteins / pharmacology*
Mice
Molecular Sequence Data
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Vero Cells
Virus Internalization / drug effects*
|
| IF |
2.985
|
| 引用数 |
37
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 5818 |
