| RRC ID |
19131
|
| 著者 |
Matsuzawa T, Ohashi T, Hosomi A, Tanaka N, Tohda H, Takegawa K.
|
| タイトル |
The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
The budding yeast Saccharomyces cerevisiae is able to utilize glycerol as the sole carbon source via two pathways (glycerol 3-phosphate pathway and dihydroxyacetone [DHA] pathway). In contrast, the fission yeast Schizosaccharomyces pombe does not grow on media containing glycerol as the sole carbon source. However, in the presence of other carbon sources such as galactose and ethanol, S. pombe could assimilate glycerol and glycerol was preferentially utilized over ethanol and galactose. No equivalent of S. cerevisiae Gcy1/glycerol dehydrogenase has been identified in S. pombe. However, we identified a gene in S. pombe, SPAC13F5.03c (gld1 (+)), that is homologous to bacterial glycerol dehydrogenase. Deletion of gld1 caused a reduction in glycerol dehydrogenase activity and prevented glycerol assimilation. The gld1 Delta cells grew on 50 mM DHA as the sole carbon source, indicating that the glycerol dehydrogenase encoded by gld1 (+) is essential for glycerol assimilation in S. pombe. Strains of S. pombe deleted for dak1 (+) and dak2 (+) encoding DHA kinases could not grow on glycerol and showed sensitivity to a higher concentration of DHA. The dak1 Delta strain showed a more severe reduction of growth on glycerol and DHA than the dak2 Delta strain because the expression of dak1 (+) mRNA was higher than that of dak2 (+). In wild-type S. pombe, expression of the gld1 (+), dak1 (+), and dak2 (+) genes was repressed at a high concentration of glucose and was derepressed during glucose starvation. We found that gld1 (+) was regulated by glucose repression and that it was derepressed in scr1 Delta and tup12 Delta strains.
|
| 巻・号 |
87(2)
|
| ページ |
715-27
|
| 公開日 |
2010-6-1
|
| DOI |
10.1007/s00253-010-2586-3
|
| PMID |
20396879
|
| MeSH |
Amino Acid Sequence
Cloning, Molecular
Fungal Proteins / chemistry
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Gene Expression Regulation, Enzymologic
Glucose / metabolism
Glycerol / metabolism*
Molecular Sequence Data
Schizosaccharomyces / chemistry
Schizosaccharomyces / enzymology*
Schizosaccharomyces / genetics
Sequence Alignment
Sugar Alcohol Dehydrogenases / chemistry
Sugar Alcohol Dehydrogenases / genetics
Sugar Alcohol Dehydrogenases / metabolism*
|
| IF |
3.53
|
| 引用数 |
34
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 酵母 |
FY18624
FY18625 |
