論文 - 詳細
| RRC ID | 19142 |
|---|---|
| 著者 | Hanaki K, Ike F, Hatakeyama R, Hirano N. |
| タイトル | Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses. |
| ジャーナル | J Virol Methods |
| Abstract |
Mouse hepatitis virus (MHV) is one of the most prevalent viruses detected in laboratory mouse colonies. Enterotropic strains predominate in natural infections, and molecular techniques for the detection of MHV shedding in feces are powerful enough to diagnose active infections. A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technique was developed for the detection of rodent coronaviruses within 90 min. The specificity of this technique was confirmed by its ability to detect all 17 different strains of MHV and 6 strains of rat coronaviruses as well as its failure to detect human, bovine, and porcine coronaviruses nonspecifically. The sensitivity of RT-LAMP was 3.2-fold higher than that of reverse transcription-polymerase chain reaction (RT-PCR) and 31.6-fold lower than that of nested RT-PCR. An evaluation of the diagnostic performance of RT-LAMP performed in duplicate using mouse fecal specimens showed that the sensitivity and specificity with respect to nested RT-PCR were 85.7% and 100%, respectively. RT-LAMP assays would be suitable for monitoring active MHV infection in mouse colonies. |
| 巻・号 | 187(2) |
| ページ | 222-7 |
| 公開日 | 2013-2-1 |
| DOI | 10.1016/j.jviromet.2012.10.008 |
| PII | S0166-0934(12)00366-7 |
| PMID | 23123121 |
| PMC | PMC7112798 |
| MeSH | Animals Coronavirus / isolation & purification* Coronavirus Infections / veterinary* Coronavirus Infections / virology Feces / virology Mice Nucleic Acid Amplification Techniques / methods* Rats Rodent Diseases / virology* Sensitivity and Specificity Veterinary Medicine / methods* Virology / methods* |
| IF | 1.786 |
| 引用数 | 5 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS VIROLOGY |
| リソース情報 | |
| 実験動物マウス | |