| RRC ID |
19148
|
| Author |
Wada H, Kojo S, Kusama C, Okamoto N, Sato Y, Ishizuka B, Seino K.
|
| Title |
Successful differentiation to T cells, but unsuccessful B-cell generation, from B-cell-derived induced pluripotent stem cells.
|
| Journal |
Int Immunol
|
| Abstract |
Forced expression of certain transcription factors in somatic cells results in generation of induced pluripotent stem (iPS) cells, which differentiate into various cell types. We investigated T-cell and B-cell lineage differentiation from iPS cells in vitro. To evaluate the impact of iPS cell source, murine splenic B-cell-derived iPS (B-iPS) cells were generated after retroviral transduction of four transcription factors (Oct4, Sox2, Klf4 and c-Myc). B-iPS cells were identical to embryonic stem (ES) cells and mouse embryonic fibroblast (MEF)-derived iPS cells in morphology, ES cell marker expression as well as teratoma and chimera mouse formation. Both B-iPS and MEF-derived iPS cells differentiated into lymphocytes in OP9 co-culture systems. Both efficiently differentiated into T-cell lineage that produced IFN-γ on T-cell receptor stimulation. However, iPS cells including B-iPS cells were relatively resistant to B-cell lineage differentiation. One of the reasons of the failure of B-cell lineage differentiation seemed due to a defect of Pax5 expression in the differentiated cells. Therefore, current in vitro differentiation systems using iPS cells are sufficient for inducing T-cell but not B-cell lineage.
|
| Volume |
23(1)
|
| Pages |
65-74
|
| Published |
2011-1-1
|
| DOI |
10.1093/intimm/dxq458
|
| PII |
dxq458
|
| PMID |
21135032
|
| MeSH |
Animals
B-Lymphocytes / cytology*
B-Lymphocytes / immunology
Cell Differentiation / immunology*
Cell Line
Cell Lineage / genetics
Coculture Techniques
CpG Islands
DNA Methylation
Gene Expression
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / immunology
Kruppel-Like Factor 4
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, SCID
PAX5 Transcription Factor / genetics
T-Lymphocytes / cytology*
T-Lymphocytes / immunology
|
| IF |
3.519
|
| Times Cited |
24
|
|
WOS Category
|
IMMUNOLOGY
|
| Resource |
| Mice |
RBRC00144 |
