論文 - 詳細
| RRC ID | 19157 |
|---|---|
| 著者 | Watanabe K, Kamata K, Sato J, Takahashi T. |
| タイトル | Fundamental studies on the inhibitory action of Acanthopanax senticosus Harms on glucose absorption. |
| ジャーナル | J Ethnopharmacol |
| Abstract |
AIM OF THE STUDY:Acanthopanax senticosus Harms extract (ASE) is used as an ingredient of over-the-counter drugs and functional foods, such as health supplements, in Japan. ASE exhibits a hypoglycemic effect; however, the mechanism of the hypoglycemic effect is not clear. In the present study, we investigated whether ASE has a glucose absorption inhibitory action. MATERIALS AND METHODS:We examined the effects of ASE on α-amylase and α-glucosidase activities, and on glucose uptake in Caco-2 cells. We also examined the effects of ASE oral administration on glucose tolerance in type 2 diabetes mellitus model db/db mice. RESULTS:The addition of ASE inhibited α-glucosidase activity but not α-amylase activity. The α-glucosidase inhibitory activity of ASE was approximately 1/13 of that of acarbose. The addition of ASE inhibited 2'-deoxy-D-glucose (DG) uptake in human intestinal Caco-2 cells, and the inhibitory activity of ASE was approximately 1/40 of that of phloretin. Kinetic analysis of glucose uptake indicated that ASE has no effects on DG uptake through passive diffusion, but that ASE inhibits intracellular DG uptake chiefly by inhibiting transport via a glucose transporter. In the glucose tolerance study, db/db mice orally administered ASE for 3 days showed significantly lower plasma glucose level than the control group 30 min after sucrose loading, without affecting plasma insulin levels. In addition, ASE oral administration significantly inhibited α-glucosidase activity in the small intestine mucosa extirpated from the mice. CONCLUSION:These findings indicate that ASE may be useful as an ingredient of functional foods to improve postprandial hyperglycemia and prevent type II diabetes mellitus. |
| 巻・号 | 132(1) |
| ページ | 193-9 |
| 公開日 | 2010-10-28 |
| DOI | 10.1016/j.jep.2010.08.014 |
| PII | S0378-8741(10)00561-1 |
| PMID | 20713144 |
| MeSH | Administration, Oral Adsorption Animals Blood Glucose / analysis Caco-2 Cells Cell Culture Techniques Diabetes Mellitus, Experimental / blood Diabetes Mellitus, Experimental / drug therapy* Diabetes Mellitus, Type 2 / blood Diabetes Mellitus, Type 2 / drug therapy* Eleutherococcus / chemistry* Glucose / metabolism* Glucose Tolerance Test Humans Hypoglycemic Agents / isolation & purification Hypoglycemic Agents / pharmacology* Hypoglycemic Agents / therapeutic use Insulin / blood Male Mice Mice, Inbred Strains Plant Bark / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plant Extracts / therapeutic use alpha-Amylases / metabolism alpha-Glucosidases / metabolism |
| IF | 3.69 |
| 引用数 | 13 |
| WOS 分野 | PLANT SCIENCES PHARMACOLOGY & PHARMACY INTEGRATIVE & COMPLEMENTARY MEDICINE CHEMISTRY, MEDICINAL |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |