Reference - Detail
| RRC ID | 19158 |
|---|---|
| Author | Wakabayashi T, Matsumine A, Nakazora S, Hasegawa M, Iino T, Ota H, Sonoda H, Sudo A, Uchida A. |
| Title | Fibulin-3 negatively regulates chondrocyte differentiation. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Fibulin-3 is a member of the fibulin family that has been newly recognized as extracellular matrix proteins. We assessed the effects of fibulin-3 overexpression on chondrocyte differentiation using the clonal murine cell line ATDC5. The ATDC5-FBLN3 stably expressing fibulin-3 protein was spindle-shaped cell compared to the ATDC5-mock with plump cell. The cell growth in the ATDC5-FBLN3 was accelerated in comparison to that in the ATDC5-mock. The ATDC5-FBLN3 was not stained by Alcian blue, nor was there any cartilage aggregate formed after the induction of chondrogenic differentiation. The expression of type II collagen, aggrecan, and type X collagen was completely suppressed in ATDC5-FBLN3 even after the induction of differentiation. The overexpression of fibulin-3 reduced the expression of Sox5 and Sox6, while it maintained the expression of Sox9. These findings suggest that fibulin-3 may play an important role as a negative regulator of chondrocyte differentiation. |
| Volume | 391(1) |
| Pages | 1116-21 |
| Published | 2010-1-1 |
| DOI | 10.1016/j.bbrc.2009.12.034 |
| PII | S0006-291X(09)02407-3 |
| PMID | 20005202 |
| MeSH | Animals Cartilage / cytology* Cartilage / metabolism Cell Differentiation* Cell Line, Tumor Chondrocytes / cytology* Chondrocytes / metabolism Chondrogenesis* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Humans Mice Proteoglycans / biosynthesis SOX9 Transcription Factor / metabolism SOXD Transcription Factors / metabolism |
| IF | 2.985 |
| Times Cited | 23 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |