Reference - Detail
| RRC ID | 19171 |
|---|---|
| Author | Tominaga A, Sugawara H, Futagawa T, Inoue K, Sasaki K, Minamino N, Hatakeyama M, Handa H, Miyata A. |
| Title | Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide (PACAP) gene. |
| Journal | Genes Cells |
| Abstract |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide localized in the testis at concentration comparable to that found in the brain, suggesting involvement in spermatogenesis. In this study, we identified the human PACAP testis-specific exon (TSE) 10.9 kb upstream from the translational start site and found that the testis-specific transcript of the human PACAP gene was found to be spliced from the TSE into a region of intron 2 without a frameshift. The resulting PACAP precursor has no signal peptide, suggesting that PACAP functions physiologically in an intracrine manner in the testis. The 5'-flanking region of the TSE contains an 80-bp fragment with potent promoter activity in testicular F9 cell. Electrophoresis mobility shift assays showed that proteins from the F9 nuclear extract interacted specifically with the 80-bp fragment. DNA affinity chromatography allowed isolation of the specific proteins bound to the 80-bp fragment, two of which were identified as Poly (ADP-ribose) polymerase-1 (PARP-1) and TIA-1-related protein (TIAR) by mass spectrometry. By using their siRNAs, the depletion of their proteins in F9 cells affected the potent promoter activity of the 80-bp fragment, suggesting that they might be involved in the testis-specific gene expression of PACAP. |
| Volume | 15(6) |
| Pages | 595-606 |
| Published | 2010-6-1 |
| DOI | 10.1111/j.1365-2443.2010.01403.x |
| PII | GTC1403 |
| PMID | 20500521 |
| MeSH | Alternative Splicing Amino Acid Sequence Animals Base Sequence Binding Sites / genetics Cell Line, Tumor Electrophoretic Mobility Shift Assay Exons / genetics Gene Expression Profiling* Humans Luciferases / genetics Luciferases / metabolism Male Mice Molecular Sequence Data Pituitary Adenylate Cyclase-Activating Polypeptide / genetics* Pituitary Adenylate Cyclase-Activating Polypeptide / metabolism Poly(ADP-ribose) Polymerases / metabolism Promoter Regions, Genetic / genetics* Protein Binding RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Swiss 3T3 Cells Testis / metabolism* Transfection |
| IF | 1.655 |
| Times Cited | 7 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NEC14(RCB0490) |