論文 - 詳細
| RRC ID | 19194 |
|---|---|
| 著者 | Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M. |
| タイトル | Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22. |
| ジャーナル | J Periodontal Res |
| Abstract |
BACKGROUND AND OBJECTIVE:Recent epidemiological studies have shown a correlation between periodontitis and hyperlipidemia. We have found high levels of oxidized low-density lipoprotein (OxLDL) in the gingival crevicular fluid of dental patients. In the present study, we tried to examine the possible role of OxLDL in periodontal inflammation in vitro. MATERIAL AND METHODS:Cells of the human gingival epithelial cell line Ca9-22 were cultured in media containing OxLDL, and the amounts of interleukin-8 (IL-8) and prostaglandin E(2) (PGE(2)) produced were measured using ELISAs. RESULTS:Production of IL-8 by Ca9-22 cells was significantly increased when the cells were treated with OxLDL, but not with native LDL or acetylated LDL. Production of PGE(2) by Ca9-22 cells was enhanced by co-incubation with OxLDL and interleukin-1 beta (IL-1 beta). Scavenger receptor inhibitors, fucoidan and dextran sulfate, inhibited the OxLDL-induced IL-8 and PGE(2) production in the presence of IL-1 beta. The p(38) MAPK inhibitors SB203580 and SB202190 and the ERK inhibitor PD98059 inhibited the OxLDL-induced IL-8 production. Among oxidized lipids and chemically modified LDL, 7-ketocholesterol enhanced IL-8 production. CONCLUSION:This is the first report to show that OxLDL enhances IL-8 production in epithelial cells. |
| 巻・号 | 45(4) |
| ページ | 488-95 |
| 公開日 | 2010-8-1 |
| DOI | 10.1111/j.1600-0765.2009.01263.x |
| PII | JRE1263 |
| PMID | 20412422 |
| MeSH | Cell Line, Tumor Chemokine CCL2 / analysis Cholesterol 7-alpha-Hydroxylase / antagonists & inhibitors Dextran Sulfate / pharmacology Dinoprostone / analysis Enzyme Inhibitors / pharmacology Epithelial Cells / drug effects Flavonoids / pharmacology Fucose / pharmacology Gingiva / cytology Gingiva / drug effects* Humans Imidazoles / pharmacology Interleukin-1beta / analysis Interleukin-1beta / pharmacology Interleukin-8 / analysis Interleukin-8 / antagonists & inhibitors Interleukin-8 / drug effects* Ketocholesterols / pharmacology Lipoproteins, LDL / antagonists & inhibitors Lipoproteins, LDL / pharmacology* Mitogen-Activated Protein Kinases / antagonists & inhibitors Oxidation-Reduction Periodontitis / metabolism Polysaccharides / pharmacology Pyridines / pharmacology Receptors, Scavenger / antagonists & inhibitors Sulfuric Acid Esters / pharmacology p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors |
| IF | 2.926 |
| 引用数 | 12 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) HO-1-u-1(RCB2102) |