RRC ID |
19198
|
Author |
Fukushima T, Ohno J, Hayakawa T, Imayoshi R, Kawaguchi M, Doi Y, Kanaya K, Mitarai M.
|
Title |
Polycationic protamine for water-insoluble complex formation with DNA.
|
Journal |
Dent Mater J
|
Abstract |
The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects.
|
Volume |
29(5)
|
Pages |
529-35
|
Published |
2010-10-1
|
DOI |
10.4012/dmj.2009-131
|
PII |
JST.JSTAGE/dmj/2009-131
|
PMID |
20733264
|
MeSH |
3T3 Cells
Animals
Anti-Bacterial Agents / pharmacology
Biocompatible Materials / chemical synthesis*
Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology
Cell Survival / drug effects
DNA / chemical synthesis*
DNA / chemistry
DNA / pharmacology
DNA Adducts / chemical synthesis
DNA Adducts / chemistry
DNA Adducts / pharmacology
Implants, Experimental
Male
Materials Testing
Mice
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Osteoblasts / drug effects
Porosity
Porphyromonas gingivalis / drug effects
Prevotella intermedia / drug effects
Protamines / chemical synthesis*
Protamines / chemistry
Protamines / pharmacology
Rats
Rats, Sprague-Dawley
Salmon
Solubility
Staphylococcus aureus / drug effects
Subcutaneous Tissue / drug effects
Subcutaneous Tissue / pathology
Surface Properties
Water
|
IF |
1.359
|
Times Cited |
17
|
WOS Category
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
Resource |
Human and Animal Cells |
MC3T3-E1(RCB1126) |