RRC ID 19204
著者 Yoshikawa K, Kokudo N, Hashimoto T, Yamamoto K, Inose T, Kimura T.
タイトル Novel phthalide compounds from Sparassis crispa (Hanabiratake), Hanabiratakelide A-C, exhibiting anti-cancer related activity.
ジャーナル Biol Pharm Bull
Abstract Sparassis crispa (SC), known as Hanabiratake in Japanese, is an edible mushroom with various medicinal properties. We isolated 3 novel phthalides, designated hanabiratakelide A (1), B (2), and C (3), from the SC fruit body. In this investigation, 3 known phthalides (4-6), ubiquinone-9, and 2 known unsaturated fatty acids were also isolated. Their structures were elucidated primarily through extensive NMR experiments. The isolated compounds 1-6 were tested for their anti-oxidant activity. The in vitro superoxide dismutase-like activity of the 3 hanabiratakelides was stronger than that of vitamin C. The compounds also exerted inhibitory effects on lipopolysaccharide-stimulated nitric oxide and prostaglandin E2 production by a murine macrophage cell line, RAW264. In addition, the growth of the colon cancer cell lines Caco-2 and colon-26 was significantly inhibited by treatment with the 3 hanabiratakelides. In vivo, the frequency of azoxymethane-induced aberrant crypt foci was reduced in SC-fed F344/N rats compared to rats fed a standard diet. In conclusion, 3 novel phthalides, hanabiratakelides, derived from SC were shown to possess anti-oxidant, anti-inflammatory, and anti-tumor activity.
巻・号 33(8)
ページ 1355-9
公開日 2010-1-1
DOI 10.1248/bpb.33.1355
PII JST.JSTAGE/bpb/33.1355
PMID 20686231
MeSH Animals Anti-Inflammatory Agents, Non-Steroidal / isolation & purification Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Anticarcinogenic Agents / isolation & purification Anticarcinogenic Agents / pharmacology* Anticarcinogenic Agents / therapeutic use Antioxidants / isolation & purification Antioxidants / pharmacology* Azoxymethane Benzofurans / chemistry Benzofurans / isolation & purification Benzofurans / pharmacology* Benzofurans / therapeutic use Caco-2 Cells Cell Proliferation / drug effects Colonic Neoplasms / chemically induced Colonic Neoplasms / pathology Colonic Neoplasms / prevention & control* Dinoprostone / biosynthesis Dose-Response Relationship, Drug Female Humans Macrophages / drug effects Macrophages / enzymology Macrophages / metabolism Magnetic Resonance Spectroscopy Mice Molecular Structure Nitric Oxide / biosynthesis Polyporales / chemistry* Rats Rats, Inbred F344 Superoxide Dismutase / metabolism
IF 1.863
引用数 29
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)