Reference - Detail
| RRC ID | 19205 |
|---|---|
| Author | Yasuda Y, Tatematsu Y, Fujii S, Maeda H, Akamine A, Torabinejad M, Saito T. |
| Title | Effect of MTAD on the differentiation of osteoblast-like cells. |
| Journal | J Endod |
| Abstract |
INTRODUCTION:The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells. METHODS:The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction. RESULTS:MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H2O2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 microg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH)2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21. CONCLUSIONS:These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H2O2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine. |
| Volume | 36(2) |
| Pages | 260-3 |
| Published | 2010-2-1 |
| DOI | 10.1016/j.joen.2009.11.002 |
| PII | S0099-2399(09)00945-5 |
| PMID | 20113785 |
| MeSH | Alkaline Phosphatase / drug effects Alkaline Phosphatase / metabolism Analysis of Variance Animals Cell Differentiation / drug effects* Cell Line Cell Survival / drug effects Citric Acid / toxicity* Doxycycline / toxicity* Drug Combinations Fibroblasts / cytology Fibroblasts / drug effects Fibroblasts / metabolism Humans Integrin-Binding Sialoprotein Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Periodontal Ligament / cytology Periodontal Ligament / drug effects* Periodontal Ligament / metabolism Polysorbates / toxicity* Root Canal Irrigants / toxicity* Sialoglycoproteins / drug effects Sialoglycoproteins / metabolism Statistics, Nonparametric |
| IF | 3.118 |
| Times Cited | 13 |
| WOS Category | DENTISTRY, ORAL SURGERY & MEDICINE |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |