| RRC ID |
19207
|
| Author |
Usami S, Motoyama S, Koyota S, Wang J, Hayashi-Shibuya K, Maruyama K, Takahashi N, Saito H, Minamiya Y, Takasawa S, Ogawa J, Sugiyama T.
|
| Title |
Regenerating gene I regulates interleukin-6 production in squamous esophageal cancer cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Regenerating gene (REG) I plays important roles in cancer cell biology. The purpose of this study was to determine whether REG I affects cytokine production in cancer cells. We transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Ialpha and Ibeta and examined its effects on cytokine expression. We found that transfecting TE-5 and TE-9 cells with REG I Ialpha and Ibeta led to significantly increased expression of interleukin (IL)-6 mRNA and protein, but it had little or no effect on expression of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, interferon-gamma, tumor necrosis factor-alpha, granulocyte-colony stimulating factor or transforming growth factor-beta1. The elevated IL-6 expression seen in REG Ialpha transfectants was silenced by small interfering RNA-mediated knockdown. These finding suggest that REG I may act through IL-6 to exert effects on squamous esophageal cancer cell biology.
|
| Volume |
392(1)
|
| Pages |
4-8
|
| Published |
2010-1-29
|
| DOI |
10.1016/j.bbrc.2009.12.129
|
| PII |
S0006-291X(09)02515-7
|
| PMID |
20056108
|
| MeSH |
Cell Line, Tumor
Esophageal Neoplasms / metabolism*
Humans
Interleukin-6 / biosynthesis*
Lithostathine / genetics
Lithostathine / metabolism*
Neoplasms, Squamous Cell / metabolism*
Transfection
|
| IF |
2.985
|
| Times Cited |
9
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
TE-5 (RCB1949)
TE-9 (RCB1988) |
