| RRC ID |
19214
|
| Author |
Hirai S, Uemura T, Mizoguchi N, Lee JY, Taketani K, Nakano Y, Hoshino S, Tsuge N, Narukami T, Yu R, Takahashi N, Kawada T.
|
| Title |
Diosgenin attenuates inflammatory changes in the interaction between adipocytes and macrophages.
|
| Journal |
Mol Nutr Food Res
|
| Abstract |
Obese adipose tissues are characterized by the enhanced infiltration of macrophages. It is considered that the paracrine loop involving monocyte chemoattractant protein-1, tumor necrosis factor-alpha, and the free fatty acid between adipocytes and macrophages establishes a vicious cycle that aggravates inflammatory changes and insulin resistance in obese adipose tissues. Diosgenin, a saponin aglycon found in a variety of plants, has anti-inflammatory properties. In the present study, we examined the effect of diosgenin on the inflammatory changes in the interaction between adipocytes and macrophages. A coculture of 3T3-L1 adipocytes and RAW 264 macrophages markedly enhanced the production of tumor necrosis factor-alpha, monocyte chemoattractant protein-1, and nitric oxide compared with the sum of their single cultures; however, treatment with diosgenin inhibited the production of these proinflammatory mediators. Diosgenin also suppressed the inflammation in RAW 264 macrophages that was induced by the conditioned medium derived from 3T3-L1 adipocytes. Furthermore, diosgenin inhibited the conditioned medium-induced degradation of inhibitor kappaB and the phosphorylation of c-jun N-terminal kinase in macrophages. These results indicate that diosgenin exhibits anti-inflammatory properties in the interaction of adipocytes and macrophages by inhibiting the inflammatory signals in macrophages. Diosgenin may be useful for ameliorating the inflammatory changes in obese adipose tissues.
|
| Volume |
54(6)
|
| Pages |
797-804
|
| Published |
2010-6-1
|
| DOI |
10.1002/mnfr.200900208
|
| PMID |
19998383
|
| MeSH |
3T3-L1 Cells
Adipocytes / drug effects*
Animals
Anti-Inflammatory Agents / pharmacology*
Cell Communication / drug effects*
Coculture Techniques
Diosgenin / pharmacology*
Fatty Acids, Nonesterified / physiology
I-kappa B Proteins / metabolism
JNK Mitogen-Activated Protein Kinases / metabolism
Macrophages / drug effects
Mice
NF-KappaB Inhibitor alpha
Phosphorylation
Toll-Like Receptor 4 / physiology
Tumor Necrosis Factor-alpha / physiology
|
| IF |
5.309
|
| Times Cited |
48
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
