論文 - 詳細
| RRC ID | 1922 |
|---|---|
| 著者 | Motokawa M, Kaku M, Tohma Y, Kawata T, Fujita T, Kohno S, Tsutsui K, Ohtani J, Tenjo K, Shigekawa M, Kamada H, Tanne K. |
| タイトル | Effects of cyclic tensile forces on the expression of vascular endothelial growth factor (VEGF) and macrophage-colony-stimulating factor (M-CSF) in murine osteoblastic MC3T3-E1 cells. |
| ジャーナル | J Dent Res |
| Abstract |
It has been reported that vascular endothelial growth factor (VEGF), expressed by osteoblasts, can induce osteoclast recruitment and thus affects bone remodeling. The purpose of this study was to investigate the effects of cyclic tensile forces on the expression of VEGF and macrophage-colony-stimulating factor (M-CSF) in osteoblastic MC3T3-E1 cells. VEGF and M-CSF gene expression and protein concentration were determined by real-time PCR and enzyme-linked immunoassay. The expression of VEGF and M-CSF mRNA in the experimental group was higher than in the control group. The increase in the concentration of VEGF and M-CSF protein in the experimental group was time-dependent. Moreover, gadolinium (an S-A channel inhibitor), but not nifedipine (L-Type Ca2+ channel blocker), treatment reduced the concentration of VEGF and M-CSF mRNA and protein in the experimental groups. These findings suggest that cyclic tensile forces increase the expression of VEGF and M-CSF in osteoblastic MC3T3-E1 cells via a stretch-activated channel (S-A channel). |
| 巻・号 | 84(5) |
| ページ | 422-7 |
| 公開日 | 2005-5-1 |
| DOI | 10.1177/154405910508400505 |
| PII | 84/5/422 |
| PMID | 15840777 |
| MeSH | 3T3 Cells Animals Calcium Channel Blockers / pharmacology Gadolinium / pharmacology Gene Expression Regulation / genetics Ion Channels / antagonists & inhibitors Macrophage Colony-Stimulating Factor / analysis* Macrophage Colony-Stimulating Factor / genetics Mice Nifedipine / pharmacology Osteoblasts / metabolism* Stress, Mechanical Time Factors Vascular Endothelial Growth Factor A / analysis* Vascular Endothelial Growth Factor A / genetics |
| IF | 4.914 |
| 引用数 | 34 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |