論文 - 詳細
| RRC ID | 19220 |
|---|---|
| 著者 | Zhang F, Wang CL, Koyama Y, Mitsui N, Shionome C, Sanuki R, Suzuki N, Mayahara K, Shimizu N, Maeno M. |
| タイトル | Compressive force stimulates the gene expression of IL-17s and their receptors in MC3T3-E1 cells. |
| ジャーナル | Connect Tissue Res |
| Abstract |
During orthodontic tooth movement, cytokines released from periodontal ligament fibroblasts and alveolar bone osteoblasts can alter the process of bone remodeling. Recently, interleukin-17 (IL-17) was found to stimulate osteoclastic resorption through osteoblasts by inducing receptor activator of nuclear factor κB ligand (RANKL) expression. However, the relationship between mechanical stress and IL-17 production by osteoblasts is not clear. Therefore, we examined the effect of compressive force on the expressions of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, and their receptors (IL-17RA, IL-17RB, IL-17RC, IL-17RD, and IL-17RE) using MC3T3-E1 cells as osteoblast-like cells. We also examined the effect of IL-17A on the expression of IL-17Rs, RANKL, macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG). The cells were cultured with or without continuous compressive force (1.0 and 2.0 g/cm(2)) for up to 24 hr. The cells were also cultured with or without IL-17A (0.1, 1.0, or 10 ng/ml) for up to 72 hr. The mRNA expressions of IL-17s and their receptors were estimated by real-time polymerase chain reaction. The expression levels of IL-17s and their receptors increased depending on the compressive force. The addition of IL-17A increased the expression of IL-17RA, IL-17RB, IL-17RC, IL-17RE, RANKL, and M-CSF, whereas it decreased OPG expression. These results indicate that compressive force induces the expression of IL-17s and their receptors in osteoblast-like cells and that IL-17s and their receptors produced in response to compressive force may affect osteoclastogenesis through the expression of RANKL, M-CSF, and OPG. |
| 巻・号 | 51(5) |
| ページ | 359-69 |
| 公開日 | 2010-10-1 |
| DOI | 10.3109/03008200903456942 |
| PMID | 20497006 |
| MeSH | 3T3 Cells Animals Compressive Strength / physiology* Gene Expression Regulation / physiology* Interleukin-17 / biosynthesis* Interleukin-17 / genetics Macrophage Colony-Stimulating Factor / biosynthesis Macrophage Colony-Stimulating Factor / genetics Mice Osteoblasts / metabolism* Osteoprotegerin / biosynthesis Osteoprotegerin / genetics RANK Ligand / biosynthesis RANK Ligand / genetics Receptors, Interleukin-17 / biosynthesis* Receptors, Interleukin-17 / genetics Stress, Mechanical* |
| IF | 2.795 |
| 引用数 | 30 |
| WOS 分野 | ORTHOPEDICS CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |