| RRC ID |
19226
|
| Author |
Ishii T, Yasuchika K, Suemori H, Nakatsuji N, Ikai I, Uemoto S.
|
| Title |
Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma.
|
| Journal |
Cancer Lett
|
| Abstract |
We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one EGFP-positive cell generated both EGFP-positive and EGFP-negative cell fractions. However, one EGFP-negative cell never produced EGFP-positive cells. The EGFP-positive cells had a greater tumorigenic potential. Only the EGFP-positive cells expressed Notch1. AFP and Notch1 expression was observed in clinical intrahepatic cholangiocarcinomas. The AFP-producing cells were suggested to be CSCs. The Notch pathway might play an important role in maintaining the CSC characteristics.
|
| Volume |
294(1)
|
| Pages |
25-34
|
| Published |
2010-8-1
|
| DOI |
10.1016/j.canlet.2010.01.019
|
| PII |
S0304-3835(10)00045-5
|
| PMID |
20149523
|
| MeSH |
Aged
Aged, 80 and over
Animals
Bile Duct Neoplasms / metabolism*
Bile Duct Neoplasms / pathology
Bile Ducts, Intrahepatic
Biomarkers / metabolism
Cell Division
Cell Line, Tumor
Cholangiocarcinoma / metabolism*
Cholangiocarcinoma / pathology
Enhancer Elements, Genetic
Female
Genes, Reporter
Genetic Vectors
Green Fluorescent Proteins / genetics
Humans
Male
Mice
Mice, Inbred NOD
Middle Aged
Neoplastic Stem Cells / metabolism*
Plasmids
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Transplantation, Heterologous
alpha-Fetoproteins / biosynthesis
alpha-Fetoproteins / genetics*
|
| IF |
7.36
|
| Times Cited |
23
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
SSP-25(RCB1293)
RBE(RCB1292) |
