RRC ID 1927
著者 Ishihara K, Yamagishi N, Hatayama T.
タイトル Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs.
ジャーナル Eur J Biochem
Abstract We have shown that sodium salicylate activates the heat shock promoter and induces the expression of heat shock proteins (hsps), with a concomitant increase in the thermotolerance of cells. To determine whether these effects are generally displayed by nonsteroidal anti-inflammatory drugs (NSAIDs), we examined the effects of a cyclooxygenase inhibitor, indomethacin, and a lipoxygenase inhibitor, nordihydroguaiaretic acid. Both inhibitors up-regulated the hsp promoter at 37 degrees C through the activation of heat shock factors, and increased cellular levels of hsps in mammalian cells, although the degree of the expression of hsps and thermotolerance of cells differed depending on the drugs. Furthermore, NSAIDs such as sodium salicylate and indomethacin suppressed the protein aggregation and apoptosis caused by an expanded polyglutamine tract in a cellular model of polyglutamine disease. These findings suggest that NSAIDs generally induce the expression of hsps in mammalian cells and may be used for the protection of cells against deleterious stressors and neurodegenerative diseases.
巻・号 271(22)
ページ 4552-8
公開日 2004-11-1
DOI 10.1111/j.1432-1033.2004.04419.x
PII EJB4419
PMID 15560796
MeSH Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology* COS Cells Cell Line Cell Survival / drug effects Chlorocebus aethiops Chromatin / chemistry Cyclooxygenase Inhibitors / pharmacology Gene Expression / drug effects Heat-Shock Proteins / biosynthesis* Heat-Shock Proteins / chemistry Heat-Shock Proteins / genetics Hot Temperature Indomethacin / pharmacology Lipoxygenase Inhibitors / pharmacology Masoprocol / pharmacology Mice Mice, Inbred C3H Microscopy, Confocal Peptides / antagonists & inhibitors* Peptides / toxicity Promoter Regions, Genetic / drug effects Sodium Salicylate / pharmacology
引用数 16
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 COS-7(RCB0539) 10T1/2(RCB0247)