| RRC ID |
19270
|
| Author |
Miki Y, Itoh T, Hirano K, Eda S, Hayashi A, Yamanaka M, Beppu M.
|
| Title |
Clearance of oxidatively damaged cells by macrophages: recognition of glycoprotein clusters by macrophage-surface nucleolin as early apoptotic cells.
|
| Journal |
Biol Pharm Bull
|
| Abstract |
The mechanism of macrophage recognition of oxidatively damaged cells was investigated. Jurkat T cells exposed to various concentrations of H(2)O(2) were bound and phagocytosed by macrophages. The cells exposed to 0.1 mM H(2)O(2) were best bound. The cell-surface ligands recognized by macrophages were suggested to be sialylpolylactosaminyl sugar chains of a major sialoglycoprotein CD43 because 1) the cell binding was inhibited by oligosaccharides containing sialylpolylactosaminyl chains, and their inhibitory activity was destroyed by a polylactosamine-cleaving enzyme endo-beta-galactosidase, and by neuraminidase; 2) the oxidized Jurkat cells pretreated with either glycosidase or with anti-CD43 antibody were not bound. The macrophage receptor involved in the binding was suggested to be cell-surface nucleolin because 1) anti-nucleolin antibody inhibited the binding; 2) nucleolin-transfected HEK293 cells bound the oxidized cells; and 3) this binding was inhibited by anti-nucleolin antibody and by anti-CD43 antibody. CD43 on oxidized Jurkat cells tended to form clusters in good accordance with their susceptibility to the macrophage binding. CD43 clustering and the oxidized-cell binding to macrophages were prevented by a caspase inhibitor Z-VAD-fmk, suggesting that the oxidized and bound cells were undergoing apoptosis. Indeed, caspase-3 activity of Jurkat cells increased by the oxidation. These results suggest that moderately oxidized cells undergo apoptosis and are recognized by macrophages as early apoptotic cells.
|
| Volume |
32(4)
|
| Pages |
564-72
|
| Published |
2009-4-1
|
| DOI |
10.1248/bpb.32.564
|
| PII |
JST.JSTAGE/bpb/32.564
|
| PMID |
19336885
|
| MeSH |
Apoptosis / physiology*
Caspases / metabolism
Cell Line
Cell Nucleus / physiology
Cell Nucleus / ultrastructure
Cytosol / physiology
Cytosol / ultrastructure
Glycoproteins / chemistry*
Humans
Jurkat Cells
Leukosialin / chemistry
Macrophages / physiology*
Membrane Proteins / chemistry
Oxidation-Reduction
Oxidative Stress / physiology*
Phosphoproteins / chemistry*
RNA-Binding Proteins / chemistry*
|
| IF |
1.863
|
| Times Cited |
8
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
THP-1(RCB1189)
293(RCB1637) |
