| RRC ID |
19274
|
| 著者 |
Tahara K, Sakai T, Yamamoto H, Takeuchi H, Hirashima N, Kawashima Y.
|
| タイトル |
Improved cellular uptake of chitosan-modified PLGA nanospheres by A549 cells.
|
| ジャーナル |
Int J Pharm
|
| Abstract |
The authors have previously developed poly(DL-lactic-co-glycolic acid) (PLGA) nanospheres (NSs) as a nanoparticulate drug carrier for pulmonary administration. The present study demonstrates that chitosan (CS)-modified PLGA NSs (CS-PLGA NSs) are preferentially taken up by human lung adenocarcinoma cells (A549). PLGA NSs prepared using a water-oil-water emulsion solvent evaporation method were surface-modified by adsorption of CS. The physicochemical parameters of PLGA NS, including average size and surface charge, were measured to identify which parameter influenced cellular uptake of PLGA NS. Uptake was confirmed using fluorescence spectrophotometry and was visualized in A549 cells with confocal laser scanning microscopy (CLSM). The cytotoxicities of non- and CS-PLGA NS systems were compared in vitro by MTS assay. Cellular uptake of PLGA NS increased with decreasing diameter to the submicron level and with CS-mediated surface modification. Cellular uptake of PLGA NS was energy dependent, as shown by a reduction in uptake at lower incubation temperatures and in hypertonic growth medium used as an inhibitor of clathrin-coated pit endocytosis. CS-PLGA NSs were taken up by A549 cells in an energy-dependent manner, suggesting a clathrin-mediated endocytic process. CS-PLGA NS demonstrated low cytotoxicity, similar to non-PLGA NS.
|
| 巻・号 |
382(1-2)
|
| ページ |
198-204
|
| 公開日 |
2009-12-1
|
| DOI |
10.1016/j.ijpharm.2009.07.023
|
| PII |
S0378-5173(09)00495-5
|
| PMID |
19646519
|
| MeSH |
Adenocarcinoma / metabolism*
Biological Transport, Active
Cell Line, Tumor
Cell Survival / drug effects
Chemistry, Pharmaceutical
Chitosan / chemistry
Chitosan / metabolism*
Chitosan / toxicity
Clathrin / metabolism
Coated Pits, Cell-Membrane / metabolism
Dose-Response Relationship, Drug
Drug Carriers*
Endocytosis
Humans
Hypertonic Solutions
Kinetics
Lactic Acid / chemistry
Lactic Acid / metabolism*
Lactic Acid / toxicity
Lung Neoplasms / metabolism*
Microscopy, Confocal
Nanospheres*
Particle Size
Polyglycolic Acid / chemistry
Polyglycolic Acid / metabolism*
Polyglycolic Acid / toxicity
Polylactic Acid-Polyglycolic Acid Copolymer
Spectrometry, Fluorescence
Surface Properties
Technology, Pharmaceutical / methods
Temperature
|
| IF |
4.845
|
| 引用数 |
127
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
