| RRC ID |
19295
|
| Author |
Okamura H, Amorim BR, Wang J, Yoshida K, Haneji T.
|
| Title |
Calcineurin regulates phosphorylation status of transcription factor osterix.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Osterix is an osteoblast-specific transcriptional factor that is essential for osteoblast differentiation and bone formation. Calcineurin regulates bone formation through modulating osteoblast differentiation. However, post-translational modification of osterix such as phosphorylation and interactions between osterix and calcineurin remains unclear. In the present study, we demonstrated that calcineurin interacted with osterix determined by immunoprecipitation assay and Western analysis. Immunocytochemical study also revealed that osterix and calcineurin were co-localized in nucleus. Deletion of calcineurin binding motif on osterix molecule disrupted osterix-calcineurin interaction. Phosphorylation status of osterix was augmented by treatment with phosphatase inhibitors, FK506 and calyculin A. In contrast, treatment of recombinant calcineurin reduced phosphorylation status of osterix. Our present study suggests that calcineurin has an important role in the function of osterix through its modification of phosphorylation.
|
| Volume |
379(2)
|
| Pages |
440-4
|
| Published |
2009-2-6
|
| DOI |
10.1016/j.bbrc.2008.12.094
|
| PII |
S0006-291X(08)02490-X
|
| PMID |
19114031
|
| MeSH |
Animals
Calcineurin / metabolism*
Cell Line
Cell Nucleus / metabolism
Humans
Immunoprecipitation
Mice
Phosphorylation
Sp7 Transcription Factor
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
2.985
|
| Times Cited |
12
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
293(RCB1637) |
