論文 - 詳細
| RRC ID | 19315 |
|---|---|
| 著者 | Ozawa H, Mutai H, Matsunaga T, Tokumaru Y, Fujii M, Sakamoto K, Tomita T, Ogawa K. |
| タイトル | Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:There have been inconsistent results regarding the contribution of the connexin family of genes to tumor cell proliferation. MATERIALS AND METHODS:We aimed to clarify the role of connexin 30 (Cx30), by transfecting three kinds of vectors that express either full length Cx30 (Cx30-Full), Cx30 devoid of C-terminal region (Cx30-DelC) or Cx30 C-terminal region (Cx30-CT), in HSC-4, a head-and-neck cancer cell line. RESULTS:Transfected Cx30-Full was localized on the plasma membrane, while Cx30-DelC and Cx30-CT was expressed in the cytoplasm or circumnuclear sites. We studied the effect on the growth rate followed by immunostaining with anti-Ki-67 (MIB-1). The MIB indices of HSC-4 cells transfected with Cx30-Full and Cx30-DelC, but not Cx30-CT were shown to be significantly higher than that of the controls. CONCLUSION:Our results demonstrated that Cx30 enhanced the proliferation of HSC-4 cells and the proliferating activity was considered to be achieved without the transport of the protein onto the plasma membrane. |
| 巻・号 | 29(6) |
| ページ | 1981-5 |
| 公開日 | 2009-6-1 |
| PII | 29/6/1981 |
| PMID | 19528455 |
| MeSH | Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology* Cell Proliferation* Connexin 30 Connexins / genetics* Connexins / metabolism Genetic Vectors Head and Neck Neoplasms / genetics* Head and Neck Neoplasms / metabolism Head and Neck Neoplasms / pathology* Humans Polymerase Chain Reaction Transfection* Tumor Cells, Cultured |
| IF | 1.994 |
| 引用数 | 10 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HSC-4(RCB1902) |