論文 - 詳細
| RRC ID | 19339 |
|---|---|
| 著者 | Nakajima S, Naruto T, Miyamae T, Imagawa T, Mori M, Nishimaki S, Yokota S. |
| タイトル | Interleukin-6 inhibits early differentiation of ATDC5 chondrogenic progenitor cells. |
| ジャーナル | Cytokine |
| Abstract |
Interleukin (IL)-6 is a causative agent of systemic juvenile idiopathic arthritis (sJIA), a chronic inflammatory disease complicated with severe growth impairment. Recent trials of anti-IL-6 receptor monoclonal antibody, tocilizumab, indicated that tocilizumab blocks IL-6/IL-6 receptor-mediated inflammation, and induces catch-up growth in children with sJIA. This study evaluates the effects of IL-6 on chondrogenesis by ATDC5 cells, a clonal murine chondrogenic cell line that provides an excellent model for studying endochondral ossification at growth plate. ATDC5 cells were examined for the expression of IL-6 receptor and gp130 by fluorescence-activated cell sorting analysis. Recombinant murine IL-6 was added to ATDC5 cultures to observe cell differentiation, using a quantitative RT-PCR for the chondrogenic differentiation markers type II collagen, aggrecan, and type X collagen. To block IL-6, the anti-mouse IL-6 receptor monoclonal antibody MR16-1 was added. As a result, the cells expressed IL-6 receptor and gp130. The expression of chondrogenic differentiation marker gene was reduced by IL-6, but this was abrogated by MR16-1. We conclude that IL-6 inhibits early chondrogenesis of ATDC5 cells suggesting that IL-6 may affect committed stem cells at a cellular level during chondrogenic differentiation of growth plate chondrocytes, and that IL-6 may be a cellular-level factor in growth impairment in sJIA. |
| 巻・号 | 47(2) |
| ページ | 91-7 |
| 公開日 | 2009-8-1 |
| DOI | 10.1016/j.cyto.2009.05.002 |
| PII | S1043-4666(09)00123-9 |
| PMID | 19535264 |
| MeSH | Animals Biomarkers / metabolism Cartilage / cytology Cell Differentiation* / drug effects Cell Line, Tumor Cell Proliferation / drug effects Chondrocytes / cytology* Chondrocytes / metabolism Chondrogenesis* / drug effects Cytokine Receptor gp130 / metabolism Gene Expression / drug effects Interleukin-6 / pharmacology* Mice Receptors, Interleukin-6 / metabolism Stem Cells / cytology Stem Cells / drug effects Stem Cells / metabolism* |
| IF | 2.952 |
| 引用数 | 28 |
| WOS 分野 | IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |