RRC ID 19344
著者 Watanabe T, Nishio H, Tanigawa T, Yamagami H, Okazaki H, Watanabe K, Tominaga K, Fujiwara Y, Oshitani N, Asahara T, Nomoto K, Higuchi K, Takeuchi K, Arakawa T.
タイトル Probiotic Lactobacillus casei strain Shirota prevents indomethacin-induced small intestinal injury: involvement of lactic acid.
ジャーナル Am J Physiol Gastrointest Liver Physiol
Abstract Inflammatory responses triggered by activation of the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4 signaling pathway are a key mechanism in nonsteroidal anti-inflammatory drug-induced enteropathy. The aim of this study was to investigate the probiotic effect of Lactobacillus casei strain Shirota (LcS) on indomethacin-induced small intestinal injury. Rats pretreated with viable LcS or heat-killed LcS once or once daily for a week were administered indomethacin by gavage to induce injury. Anti-inflammatory effects of L-lactic acid (1-15 mM) were evaluated in vitro by use of THP-1 cells. One-week treatment with viable LcS prevented indomethacin-induced intestinal injury with increase in the concentration of lactic acid in small intestinal content and inhibited increases in myeloperoxidase activity and expression of mRNA for tumor necrosis factor-alpha (TNF-alpha) while affecting neither TLR4 expression nor the number of gram-negative bacteria in intestinal content, whereas neither heat-killed LcS nor a single dose of viable LcS inhibited intestinal injury. Prevention of this injury was also observed in rats given l-lactic acid in drinking water. Both L-lactic acid and LcS culture supernatant containing 10 mM lactic acid inhibited NF-kappaB activation and increases in TNF-alpha mRNA expression and TNF-alpha protein secretion in THP-1 cells treated with LPS. Western blot analyses showed that both L-lactic acid and LcS culture supernatants suppressed phosphorylation and degradation of I-kappaB-alpha induced by LPS without affecting expression of TLR4. These findings suggest that LcS exhibits a prophylactic effect on indomethacin-induced enteropathy by suppressing the LPS/TLR4 signaling pathway and that this probiotic effect of LcS may be mediated by L-lactic acid.
巻・号 297(3)
ページ G506-13
公開日 2009-9-1
DOI 10.1152/ajpgi.90553.2008
PII 90553.2008
PMID 19589943
MeSH Animals Cell Line Disease Models, Animal Humans I-kappa B Proteins / metabolism Indomethacin Intestinal Diseases / chemically induced Intestinal Diseases / metabolism Intestinal Diseases / microbiology Intestinal Diseases / prevention & control* Intestine, Small / metabolism Intestine, Small / microbiology* Intestine, Small / pathology Lactic Acid / administration & dosage Lactic Acid / metabolism* Lactobacillus casei / isolation & purification Lactobacillus casei / metabolism* Male Monocytes / metabolism NF-KappaB Inhibitor alpha NF-kappa B / metabolism Peroxidase / metabolism Phosphorylation Probiotics* RNA, Messenger / metabolism Rats Rats, Wistar Toll-Like Receptor 4 / metabolism Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / metabolism
IF 3.725
引用数 93
WOS 分野 PHYSIOLOGY GASTROENTEROLOGY & HEPATOLOGY
リソース情報
ヒト・動物細胞 THP-1(RCB1189)