| RRC ID |
19350
|
| 著者 |
Matsuyama K, Villareal MO, El Omri A, Han J, Kchouk ME, Isoda H.
|
| タイトル |
Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells.
|
| ジャーナル |
J Nat Med
|
| Abstract |
The effect of Tunisian Capparis spinosa L. aromatic plant extract on melanogenesis regulation in B16 murine melanoma cells was investigated. B16 cells were treated with 0.0005, 0.005, and 0.05% (w/v) C. spinosa extract after which the melanin content and cell viability were measured. To clarify the mechanism behind melanogenesis regulation, the expression of tyrosinase was determined. Results showed that the extract had a significant stimulative effect on melanogenesis in B16 cells in a dose-dependent manner without cytotoxicity. Western blot analysis showed that expression of tyrosinase in cells treated with 0.03% (w/v) C. spinosa extract increased by 12.5- and 20-fold after 24 and 48 h of incubation, respectively, compared with untreated cells. HPLC analysis of the extract revealed the presence of 1% quercetin, a known melanogenesis stimulator, indicating that our findings may be attributed to quercetin; however, other compounds present in the extract may also have an effect on the overall ability of the extract to stimulate melanogenesis. We report here that Tunisian C. spinosa leaf extract can stimulate melanogenesis in a dose-dependent manner without cytotoxicity by increasing tyrosinase protein expression and has the potential to be used as a possible tanning agent or as a treatment for hair depigmentation.
|
| 巻・号 |
63(4)
|
| ページ |
468-72
|
| 公開日 |
2009-10-1
|
| DOI |
10.1007/s11418-009-0355-3
|
| PMID |
19685105
|
| MeSH |
Animals
Blotting, Western
Capparis / chemistry*
Cell Line, Tumor
Cell Survival / drug effects
Chromatography, High Pressure Liquid
Gene Expression Regulation, Enzymologic / drug effects*
Melanins / biosynthesis*
Melanoma, Experimental
Mice
Monophenol Monooxygenase / metabolism
Plant Extracts / pharmacology*
Tunisia
|
| IF |
2.055
|
| 引用数 |
12
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MEDICINAL
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma 4A5(RCB0557) |
