RRC ID 19359
Author Kishimoto KN, Oxford CL, Reddi AH.
Title Stimulation of the side population fraction of ATDC5 chondroprogenitors by hypoxia.
Journal Cell Biol. Int.
Abstract The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transporters, abcb1a/b (mdr1a/b) and abcg2 (bcrp1) was quantified by RT-PCR. SP cells were characterized by the higher expression of abcb1a. The expression levels of abcb1b and abcg2 were higher than abcb1a. However, there was no significant difference between SP and non-SP fractions in the expression of abcb1b and abcg2. The telomeric repeat amplification protocol assay showed that SP cells tended to show lower telomerase activity than non-SP cells. Chondrogenic properties of ATDC5 cells derived from SP or non-SP were assessed by micromass culture. There were not significant differences between SP and non-SP derived cells in Alcian blue staining and sox9, Aggrecan, Col2a1 and SZP mRNA expression. The results demonstrate that the SP fraction was stimulated by hypoxia and chondrogenic properties of SP and non-SP fraction of ATDC5 cells were similar.
Volume 33(12)
Pages 1222-9
Published 2009-12
DOI 10.1016/j.cellbi.2009.06.009
PII S1065-6995(09)00143-7
PMID 19524690
MeSH Animals Benzimidazoles Cell Differentiation Cell Fractionation Cell Hypoxia / physiology* Cell Line, Tumor Cell Proliferation Chondrocytes / metabolism Chondrocytes / pathology* Extracellular Matrix / metabolism Fluorescent Dyes Gene Expression Mice Oxygen / physiology Stem Cells / metabolism Stem Cells / pathology* Telomerase / metabolism*
IF 2.127
Times Cited 5
Human and Animal Cells ATDC5 (RCB0565)