RRC ID 19361
著者 Kumagishi K, Nishida K, Yamaai T, Momota R, Miyaki S, Hirohata S, Naito I, Asahara H, Ninomiya Y, Ohtsuka A.
タイトル A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) expression by chondrocytes during endochondral ossification.
ジャーナル Arch Histol Cytol
Abstract A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) is known to influence aggrecan degradation in endochondral ossification, but its role has not been well understood. In the present study, in vitro gene expression of ADAMTS9 was investigated by RT-PCR in ATDC5 cells in which experimentally chondrogenic differentiation had been induced. We also investigated the protein localization and gene expression pattern of ADAMTS9 in the tibia growth plate cartilage of male mice in a day 1 neonate, 7-week-old young adult, and a 12-week-old adult by immunohistochemistry and in situ hybridization and compared the results with the expression of proliferating cell nuclear antigen (PCNA) and type X collagen for the identification of proliferative and hypertrophic chondrocyte phenotypes, respectively. We found the gene expression of ADAMTS9 by ATDC5 cells as a dual mode, both before the expression of type X collagen and after hypertrophic differentiation. The immunoreactivity of ADAMTS9 was observed in chondrocytes of proliferative and hypertrophic zones in the growth plate. The population of ADAMTS9 positive cells decreased with age. The results of the present study suggest that ADAMTS9 might have a role in aggrecan cleavage around the chondrocytes to allow chondrocyte proliferation and hypertrophy.
巻・号 72(3)
ページ 175-85
公開日 2009-1-1
DOI 10.1679/aohc.72.175
PII JST.JSTAGE/aohc/72.175
PMID 20513980
MeSH ADAM Proteins / genetics ADAM Proteins / metabolism* ADAMTS9 Protein Age Factors Animals Cell Line Cell Proliferation Chondrocytes / metabolism* Collagen Type X / metabolism Male Mice Mice, Inbred ICR Osteogenesis* Proliferating Cell Nuclear Antigen / metabolism Tibia / metabolism
引用数 6
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)