論文 - 詳細
| RRC ID | 19365 |
|---|---|
| 著者 | Nakamura T, Kodama N, Oda M, Tsuchiya S, Arai Y, Kumamoto T, Ishikawa T, Ueno K, Yano S. |
| タイトル | The structure-activity relationship between oxycoumarin derivatives showing inhibitory effects on iNOS in mouse macrophage RAW264.7 cells. |
| ジャーナル | J Nat Med |
| Abstract |
We have investigated the structure-activity relationship between 63 natural oxycoumarin derivatives and their effects on the expression of inducible-nitric oxide synthase (iNOS) induced by lipopolysaccharide. The protein expression of iNOS was screened by Western blot analysis, and four 5,7-dimethoxycoumarins were selected as potent inhibitors of iNOS expression. In terms of structural specificity, the methoxyl group on C-5 and C-7 and the short alkyl chain (1-5 carbons) on C-6 may be essential for the potent activities. These compounds also showed inhibitory effects on nitric oxide generation and mRNA expression of inflammatory mediators, namely, iNOS and COX-2. Interestingly, the inhibitory effect on mRNA expression was specific for iNOS and was not detected for neuronal NOS. It is expected that these compounds will show anti-inflammatory activities via inhibition of the expressions of iNOS and COX-2. |
| 巻・号 | 63(1) |
| ページ | 15-20 |
| 公開日 | 2009-1-1 |
| DOI | 10.1007/s11418-008-0268-6 |
| PMID | 18607531 |
| MeSH | Animals Blotting, Western Cell Line Coumarins / chemistry Coumarins / pharmacology* Lipopolysaccharides / pharmacology Macrophages / cytology Macrophages / drug effects* Macrophages / enzymology Mice Molecular Structure Nitric Oxide / metabolism Nitric Oxide Synthase Type II / antagonists & inhibitors* Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / metabolism Reverse Transcriptase Polymerase Chain Reaction Structure-Activity Relationship |
| IF | 2.055 |
| 引用数 | 30 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |