Reference - Detail
RRC ID | 19380 |
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Author | Matsuzawa T, Hara F, Tanaka N, Tohda H, Takegawa K. |
Title | Ght2⁺ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe. |
Journal | Appl Microbiol Biotechnol |
Abstract |
Schizosaccharomyces pombe has eight hexose transporter genes, ght1 (+) to ght8 (+). Here we report that ght2 (+), which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 (+) in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose. |
Volume | 97(11) |
Pages | 4957-64 |
Published | 2013-6-1 |
DOI | 10.1007/s00253-012-4637-4 |
PMID | 23254763 |
MeSH | Culture Media / chemistry Galactose / metabolism* Glucose / metabolism Monosaccharide Transport Proteins / genetics Monosaccharide Transport Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Uridine Diphosphate Galactose / biosynthesis* |
IF | 3.53 |
Times Cited | 0 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
Resource | |
Yeast | FY18625 FY18624 |