論文 - 詳細
| RRC ID | 1942 |
|---|---|
| 著者 | Luo JC, Shin VY, Yang YH, Wu WK, Ye YN, So WH, Chang FY, Cho CH. |
| タイトル | Tumor necrosis factor-alpha stimulates gastric epithelial cell proliferation. |
| ジャーナル | Am J Physiol Gastrointest Liver Physiol |
| Abstract |
TNF-alpha is a cytokine produced during gastric mucosal injury. We examined whether TNF-alpha could promote mucosal repair by stimulation of epithelial cell proliferation and explored further the underlying mechanisms in a rat gastric mucosal epithelial cell line (RGM-1). TNF-alpha treatment (1-10 ng/ml) for 12 or 24 h significantly increased cell proliferation but did not induce apoptosis in RGM-1 cells. TNF-alpha treatment significantly increased cytosolic phospholipase A(2) and cyclooxygenase-2 (COX-2) protein expression and PGE(2) level but did not affect the protein levels of EGF, basic fibroblast growth factor, and COX-1 in RGM-1 cells. The mRNA of TNF receptor (TNF-R) 2 but not of TNF-R1 was also increased. Dexamethasone dose dependently inhibited the stimulatory effect of TNF-alpha on cell proliferation, which was associated with a significant decrease in cellular COX-2 expression and PGE(2) level. A selective COX-2 inhibitor 3-(3-fluorophenyl)-4-[4-(methylsulfonyl)phenyl]-5,5-dimethyl-(5)H-furan-2-one (DFU) by itself had no effect on basal cell proliferation but significantly reduced the stimulatory effect of TNF-alpha on RMG-1 cells. Combination of dexamethasone and DFU did not produce an additive effect. PGE(2) significantly reversed the depressive action of dexamethasone on cell proliferation. These results suggest that TNF-alpha plays a regulatory role in epithelial cell repair in the gastric mucosa via the TNF-alpha receptor and activation of the arachidonic acid/PG pathway. |
| 巻・号 | 288(1) |
| ページ | G32-8 |
| 公開日 | 2005-1-1 |
| DOI | 10.1152/ajpgi.00093.2004 |
| PII | 00093.2004 |
| PMID | 15256360 |
| MeSH | Animals Apoptosis Arachidonic Acid / pharmacology Cell Proliferation* Cell Survival Cyclooxygenase 2 Dinoprostone / pharmacology Epithelial Cells / physiology Gastric Mucosa / cytology* Gastric Mucosa / pathology* Gene Expression Regulation Prostaglandin-Endoperoxide Synthases / biosynthesis Rats Stomach / cytology* Stomach / physiology Tumor Necrosis Factor-alpha / pharmacology* |
| IF | 3.725 |
| 引用数 | 25 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY PHYSIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |