| RRC ID |
1942
|
| 著者 |
Luo JC, Shin VY, Yang YH, Wu WK, Ye YN, So WH, Chang FY, Cho CH.
|
| タイトル |
Tumor necrosis factor-alpha stimulates gastric epithelial cell proliferation.
|
| ジャーナル |
Am J Physiol Gastrointest Liver Physiol
|
| Abstract |
TNF-alpha is a cytokine produced during gastric mucosal injury. We examined whether TNF-alpha could promote mucosal repair by stimulation of epithelial cell proliferation and explored further the underlying mechanisms in a rat gastric mucosal epithelial cell line (RGM-1). TNF-alpha treatment (1-10 ng/ml) for 12 or 24 h significantly increased cell proliferation but did not induce apoptosis in RGM-1 cells. TNF-alpha treatment significantly increased cytosolic phospholipase A(2) and cyclooxygenase-2 (COX-2) protein expression and PGE(2) level but did not affect the protein levels of EGF, basic fibroblast growth factor, and COX-1 in RGM-1 cells. The mRNA of TNF receptor (TNF-R) 2 but not of TNF-R1 was also increased. Dexamethasone dose dependently inhibited the stimulatory effect of TNF-alpha on cell proliferation, which was associated with a significant decrease in cellular COX-2 expression and PGE(2) level. A selective COX-2 inhibitor 3-(3-fluorophenyl)-4-[4-(methylsulfonyl)phenyl]-5,5-dimethyl-(5)H-furan-2-one (DFU) by itself had no effect on basal cell proliferation but significantly reduced the stimulatory effect of TNF-alpha on RMG-1 cells. Combination of dexamethasone and DFU did not produce an additive effect. PGE(2) significantly reversed the depressive action of dexamethasone on cell proliferation. These results suggest that TNF-alpha plays a regulatory role in epithelial cell repair in the gastric mucosa via the TNF-alpha receptor and activation of the arachidonic acid/PG pathway.
|
| 巻・号 |
288(1)
|
| ページ |
G32-8
|
| 公開日 |
2005-1-1
|
| DOI |
10.1152/ajpgi.00093.2004
|
| PII |
00093.2004
|
| PMID |
15256360
|
| MeSH |
Animals
Apoptosis
Arachidonic Acid / pharmacology
Cell Proliferation*
Cell Survival
Cyclooxygenase 2
Dinoprostone / pharmacology
Epithelial Cells / physiology
Gastric Mucosa / cytology*
Gastric Mucosa / pathology*
Gene Expression Regulation
Prostaglandin-Endoperoxide Synthases / biosynthesis
Rats
Stomach / cytology*
Stomach / physiology
Tumor Necrosis Factor-alpha / pharmacology*
|
| IF |
3.725
|
| 引用数 |
25
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
PHYSIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
