| RRC ID |
1956
|
| Author |
Kodama N, Kambayashi Y, Kubo M, Nobukuni Y, Kimura S, Nakamura H, Ogino K.
|
| Title |
Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1.
|
| Journal |
Cell Biochem Funct
|
| Abstract |
The human eosinophilic leukemia cell line, EoL-1, differentiated with butyrate as an eosinophilic cellular model was evaluated for peroxidase-dependent tyrosine nitration. Butyrate suppressed cell growth and induced eosinophilic granules in EoL-1 cells after 9 days of culture. Peroxidase activity was detected biochemically and histochemically from 3-day cultures and it increased in a time dependent manner. This peroxidase activity was inhibited by cyanide. Nitrotyrosine formation catalysed by peroxidase using hydrogen peroxide and nitrite was detected at a high level similar to that of mature eosinophils. However, no expression of eosinophil peroxidase (EPO) was detected by RT-PCR or immunocytochemistry. In contrast, the induction of myeloperoxidase (MPO) by butyrate was clearly detected by RT-PCR, Northern blot, and immunocytochemical staining. These results suggest that butyrate induces MPO rather than EPO in EoL-1 cells and that the formation of nitrotyrosine in butyrate-induced cells is dependent on MPO.
|
| Volume |
22(2)
|
| Pages |
105-12
|
| Published |
2004-1-1
|
| DOI |
10.1002/cbf.1051
|
| PMID |
15027099
|
| MeSH |
Butyrates / pharmacology
Cell Differentiation / drug effects
Chromatography, High Pressure Liquid
Cytoplasmic Granules / drug effects
Eosinophil Peroxidase
Humans
Hypereosinophilic Syndrome / drug therapy
Hypereosinophilic Syndrome / enzymology*
Hypereosinophilic Syndrome / metabolism
Immunohistochemistry
Peroxidase / metabolism*
Peroxidases / metabolism
Tyrosine / analogs & derivatives*
Tyrosine / biosynthesis*
|
| IF |
2.632
|
| Times Cited |
2
|
|
WOS Category
|
CELL BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
EoL-1 cell(RCB0641) |
