RRC ID 1956
Author Kodama N, Kambayashi Y, Kubo M, Nobukuni Y, Kimura S, Nakamura H, Ogino K.
Title Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1.
Journal Cell Biochem Funct
Abstract The human eosinophilic leukemia cell line, EoL-1, differentiated with butyrate as an eosinophilic cellular model was evaluated for peroxidase-dependent tyrosine nitration. Butyrate suppressed cell growth and induced eosinophilic granules in EoL-1 cells after 9 days of culture. Peroxidase activity was detected biochemically and histochemically from 3-day cultures and it increased in a time dependent manner. This peroxidase activity was inhibited by cyanide. Nitrotyrosine formation catalysed by peroxidase using hydrogen peroxide and nitrite was detected at a high level similar to that of mature eosinophils. However, no expression of eosinophil peroxidase (EPO) was detected by RT-PCR or immunocytochemistry. In contrast, the induction of myeloperoxidase (MPO) by butyrate was clearly detected by RT-PCR, Northern blot, and immunocytochemical staining. These results suggest that butyrate induces MPO rather than EPO in EoL-1 cells and that the formation of nitrotyrosine in butyrate-induced cells is dependent on MPO.
Volume 22(2)
Pages 105-12
Published 2004-1-1
DOI 10.1002/cbf.1051
PMID 15027099
MeSH Butyrates / pharmacology Cell Differentiation / drug effects Chromatography, High Pressure Liquid Cytoplasmic Granules / drug effects Eosinophil Peroxidase Humans Hypereosinophilic Syndrome / drug therapy Hypereosinophilic Syndrome / enzymology* Hypereosinophilic Syndrome / metabolism Immunohistochemistry Peroxidase / metabolism* Peroxidases / metabolism Tyrosine / analogs & derivatives* Tyrosine / biosynthesis*
IF 2.632
Times Cited 2
Human and Animal Cells EoL-1 cell(RCB0641)