論文 - 詳細
| RRC ID | 196 |
|---|---|
| 著者 | Wang A, Nishihashi T, Trandafir CC, Murakami S, Ji X, Shimizu Y, Kurahashi K. |
| タイトル | Involvement of endothelial cyclo-oxygenase metabolites in noradrenaline-induced contraction of rat coronary artery. |
| ジャーナル | Clin Exp Pharmacol Physiol |
| Abstract |
1. Noradrenaline (NA; 0.3 micromol/L) caused a contraction of the rat coronary artery that markedly increased in the presence of the nitric oxide synthase (NOS) inhibitor N(G)-nitro-l-arginine methyl ester (L-NAME; 100 micromol/L) and arachidonic acid (1 micromol/L; P < 0.05). 2. The present experiments attempted to elucidate the endothelium dependency of the contraction and to pharmacologically characterize the factors involved in the contraction induced by NA (0.3 micromol/L) in the presence of L-NAME and arachidonic acid in ring preparations of the rat coronary artery. 3. The NA (0.3 micromol/L)-induced contraction was attenuated by a chemical remover of the endothelium (saponin at concentrations of 0.1 and 0.4 mg/mL) in a concentration-dependent manner (P < 0.05). 4. The cyclo-oxygenase (COX)-1 inhibitor flurbiprofen (0.01-1 micromol/L) and the COX-2 inhibitor nimesulide (0.01-1 micromol/L) attenuated the NA-induced contraction in a concentration-dependent manner and the inhibitory effect of flurbiprofen was significantly more potent than that of nimesulide (P < 0.05). The 5-lipoxigenase inhibitor ZM-230487 (1 micromol/L) did not affect the NA-induced contraction. 5. The thromboxane A2 (TXA2) synthetase inhibitor OKY-046 (30 micromol/L) and the TXA2 antagonist S-1452 (0.1-10 micromol/L) did not attenuate the NA-induced contraction. 6. These results indicate that the contraction induced by NA in the rat coronary artery in the presence of L-NAME and arachidonic acid is endothelium dependent and is due to endothelial COX metabolites of arachidonic acid. |
| 巻・号 | 32(8) |
| ページ | 628-32 |
| 公開日 | 2005-8-1 |
| DOI | 10.1111/j.0305-1870.2005.04242.x |
| PII | CEP4242 |
| PMID | 16120189 |
| MeSH | Animals Coronary Vessels / drug effects* Coronary Vessels / metabolism Coronary Vessels / physiology* Cyclooxygenase Inhibitors / pharmacology Dose-Response Relationship, Drug Endothelium, Vascular / enzymology* Lipoxygenase Inhibitors / pharmacology Male Norepinephrine / pharmacology* Prostaglandin-Endoperoxide Synthases / metabolism* Rats Rats, Wistar Thromboxane A2 / antagonists & inhibitors Vasoconstriction / drug effects* |
| IF | 2.456 |
| 引用数 | 10 |
| WOS 分野 | PHARMACOLOGY & PHARMACY PHYSIOLOGY |
| リソース情報 | |
| ラット | |