| RRC ID |
1960
|
| Author |
Xie Q, Matsunaga S, Niimi S, Ogawa S, Tokuyasu K, Sakakibara Y, Machida S.
|
| Title |
Human lectin-like oxidized low-density lipoprotein receptor-1 functions as a dimer in living cells.
|
| Journal |
DNA Cell Biol
|
| Abstract |
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a unique scavenger receptor that plays important roles in atherogenesis and has been thought to function as a monomer. Using coimmunoprecipitation studies, we demonstrate that human LOX-1 (hLOX-1) forms constitutive homo-interactions in vivo. Western blot analysis of cell lysates under nonreducing or reducing conditions revealed one clear immunoreactive species corresponding to the size of a putative receptor dimer or a monomer, respectively, consistent with the presence of disulfide-linked hLOX-1 complexes. Site-directed mutagenesis studies indicated that cysteine 140 has a key role in the formation of these disulfide-linked hLOX-1 dimers. Eliminating this intermolecular disulfide bond markedly impairs the recognition of Escherichia coli by hLOX-1. Furthermore, these dimers can act as a "structural unit" to form noncovalently associated oligomers, as demonstrated by a membrane-impermeant crosslinker, which resulted in immunoreactive species corresponding to the sizes of putative tetramers and hexamers. These results provide the first evidence for the existence of hLOX-1 dimers/oligomers.
|
| Volume |
23(2)
|
| Pages |
111-7
|
| Published |
2004-2-1
|
| DOI |
10.1089/104454904322759920
|
| PMID |
15000751
|
| MeSH |
Animals
Blotting, Western
COS Cells
Chlorocebus aethiops
DNA, Complementary / genetics
Dimerization
Fluorescent Antibody Technique
Humans
Mutagenesis, Site-Directed
Plasmids / genetics
Precipitin Tests
Receptors, LDL / genetics*
Receptors, LDL / metabolism*
|
| IF |
3.314
|
| Times Cited |
39
|
|
WOS Category
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
