RRC ID 19612
著者 Li X, Liu X, Dong W, Feng Q, Cui F, Uo M, Akasaka T, Watari F.
タイトル In vitro evaluation of porous poly(L-lactic acid) scaffold reinforced by chitin fibers.
ジャーナル J Biomed Mater Res B Appl Biomater
Abstract In this study, the previously reported porous three-dimensional poly(L-lactic acid) (PLLA) scaffolds reinforced by the chitin fibers (PLLA/CF) with and without the link were evaluated in vitro. Firstly, pH value of the phosphate buffered saline lixiviums of the PLLA/CF with different content of the chitin fibers was measured to get an appropriate content of the chitin fibers in the PLLA/CF. Then, the cell functions (attachment, proliferation, alkaline phosphatase per unit cell, total protein per unit cell, and osteonectin, osteopontin, and osteocalcin gene expression) of human osteoblast-like cells (SaOS2) cultured on the PLLA/CF with the link, PLLA/CF without the link and PLLA scaffold were compared. The results showed that the link treatment did not significantly influence the pH value of the lixiviums of the scaffolds, 30% volume content might be an appropriate content of the chitin fibers in PLLA/CF scaffold to keep the pH value of the lixiviums of the scaffolds between 7.0 and 7.2 during the lixiviation time of 16 weeks, the PLLA/CF scaffold was significantly better for the attachment, proliferation, differentiation, and mineralization of the osteoblast than PLLA, the link treatment did not significantly influence these cells activities, which further suggested that PLLA/CF with the link treatment might be an appropriate scaffold for tissue engineering.
巻・号 90(2)
ページ 503-9
公開日 2009-8-1
DOI 10.1002/jbm.b.31311
PMID 19145630
MeSH Alkaline Phosphatase / metabolism Biocompatible Materials / chemistry* Cells, Cultured / cytology Chitin / chemistry* Gene Expression Regulation Humans Hydrogen-Ion Concentration Lactic Acid / chemistry* Osteoblasts / cytology Osteoblasts / metabolism Osteocalcin / metabolism Osteonectin / metabolism Osteopontin / metabolism Phosphates / chemistry Polyesters Polymers / chemistry* Porosity Tissue Engineering / methods Tissue Scaffolds / chemistry*
IF 2.831
引用数 49
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 Saos-2(RCB0428)