論文 - 詳細
| RRC ID | 19622 |
|---|---|
| 著者 | Kobayashi K, Yasuda Y, Shintani Y, Sumitomo T, Saga T, Kimura M, Yamamoto A, Mori T, Maeda A, Yamaguchi Y, Morita A. |
| タイトル | The development of a filter to enhance the efficacy and safety of excimer light (308 nm) therapy. |
| ジャーナル | Photodermatol Photoimmunol Photomed |
| Abstract |
BACKGROUND:Excimer light (308 nm) therapy is a new ultraviolet (UV) B phototherapy for which the efficacy and resulting DNA damage are not well established. PURPOSE:To develop an effective and safe phototherapy using the excimer lamp, we studied the effects of different light cut-off filters, A and B. METHODS:Efficacy was evaluated by measuring apoptosis using fluorescence-activated cell sorting analysis. DNA damage was evaluated by measuring cyclobutane pyrimidine dimers (CPDs). Light sources, including normal wave and short wave (SW) excimer light, broad-band (BB) UVB, and narrow-band (NB) UVB, were examined using the filters. A human skin equivalent model was also examined. RESULTS:The ratio of positive apoptosis to CPD formation normalized to the mean induced by NB-UVB was 5.7 using the excimer lamp without a filter, 6.3 using the excimer lamp with the A filter, 6.4 using the SW excimer lamp without a filter, and 4.2 using the BB-UVB. The A filter reduced CPD formation induced by the normal wave and SW excimer lamp. In the human skin equivalent model, the use of filters significantly decreased the amount of CPD-positive cells. CONCLUSIONS:These findings suggest that using the A filter with the excimer lamp increases the efficacy and safety of excimer light therapy. |
| 巻・号 | 25(1) |
| ページ | 30-6 |
| 公開日 | 2009-2-1 |
| DOI | 10.1111/j.1600-0781.2009.00396.x |
| PII | PPP396 |
| PMID | 19152513 |
| MeSH | Apoptosis / radiation effects Cell Line Filtration / instrumentation* Humans Models, Biological Pyrimidine Dimers / metabolism Skin / metabolism Skin / radiation effects Ultraviolet Rays Ultraviolet Therapy / instrumentation* Ultraviolet Therapy / methods* |
| IF | 2.387 |
| 引用数 | 11 |
| WOS 分野 | DERMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB0806) |