RRC ID 19640
著者 Tiğli RS, Gümüşderelioğlu M.
タイトル Evaluation of alginate-chitosan semi IPNs as cartilage scaffolds.
ジャーナル J Mater Sci Mater Med
Abstract In this study, alginate and alginate:chitosan semi interpenetrating polymer network (IPN) scaffolds were prepared by freeze-drying process. Alginate scaffolds were crosslinked with different concentrations of CaCl(2), i.e. 0.5, 1 or 3% (w/v), in 96% (v/v) ethanol solutions for two different periods, i.e. 4 and 24 h, after freeze-drying. Scanning electron microscope (SEM)/ Energy Dispersive Analysis by X-ray (EDAX) analysis and swelling studies indicated that crosslinking of scaffolds with 3% (w/v) CaCl(2) for 24 h was effectively created suitable alginate scaffolds in terms of optimum porosity and mechanical stability. This is why, alginate:chitosan semi IPN scaffolds were prepared at the crosslinking condition mentioned above in 70:30, 60:40 and 50:50% (v/v) alginate:chitosan ratios. Besides the attachment and proliferation abilities of ATDC5 murine chondrogenic cells on alginate, 70:30% (v/v) alginate:chitosan and 50:50% (v/v) alginate:chitosan scaffolds, their cellular responses were assessed for chondrogenic potential. These structural and cellular outcomes demonstrate potential utility of chitosan semi IPNs in alginate scaffolds. Comparative results found in relation to alginate scaffolds, support the necessity for alginate:chitosan scaffolds for improved cartilage tissue engineering.
巻・号 20(3)
ページ 699-709
公開日 2009-3-1
DOI 10.1007/s10856-008-3624-x
PMID 18987950
MeSH Alginates / chemistry* Animals Cell Adhesion Cell Line Cell Proliferation Cell Survival Chitosan / chemistry* Chondrocytes / cytology Chondrogenesis Cross-Linking Reagents Freeze Drying Glucuronic Acid / chemistry Hexuronic Acids / chemistry Materials Testing Mice Microscopy, Electron, Scanning Tissue Engineering Tissue Scaffolds / chemistry*
IF 2.489
引用数 31
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)