論文 - 詳細
| RRC ID | 19651 |
|---|---|
| 著者 | Tiğli RS, Gümüşderelioğlu M. |
| タイトル | Chondrogenesis on BMP-6 loaded chitosan scaffolds in stationary and dynamic cultures. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffolds and cultured for 28 days under static conditions. In the following part, we examined the influence of dynamic cultivation conditions over BMP-6 loaded chitosan scaffolds by using rotating bioreactor with perfusion (RCMW). Tissue engineered constructs were characterized by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) assay, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and biochemical assays for glycosaminoglycans (GAG) deoxyribonucleic acid (DNA) and collagen Type II quantification. At the end of 4 weeks static incubation period high levels of GAG (21.22 mg/g dry weight), DNA amounts (1.37 mg/g dry weight) and collagen Type II amounts (1.94 microg/g dry weight) were achieved for BMP-6 loaded chitosan scaffolds compared to chitosan scaffolds. However, the results obtained from morphological observations suggested hypertrophic differentiation of ATDC5 cells in the presence of BMP-6 under stationary conditions. The influence of mechanical stimulation appeared significantly with differentiated cells, cultured under dynamic conditions, showing the effect of retaining their phenotypes without hypertrophy. |
| 巻・号 | 104(3) |
| ページ | 601-10 |
| 公開日 | 2009-10-15 |
| DOI | 10.1002/bit.22426 |
| PMID | 19575410 |
| MeSH | Animals Bone Morphogenetic Protein 6 / metabolism* Cell Survival Chitosan* Chondrocytes / drug effects* Chondrocytes / ultrastructure Chondrogenesis* Collagen Type II / analysis DNA / analysis Glycosaminoglycans / analysis Mice Microscopy, Confocal Microscopy, Electron, Scanning Tetrazolium Salts / metabolism Thiazoles / metabolism Tissue Engineering / methods* |
| IF | 4.002 |
| 引用数 | 15 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |