Reference - Detail
| RRC ID | 19663 |
|---|---|
| Author | Bak EJ, Ishii Y, Omatsu T, Kyuwa S, Tetsuya T, Hayasaka I, Yoshikawa Y. |
| Title | Identification and analysis of MHC class II DRB1 (Patr-DRB1) alleles in chimpanzees. |
| Journal | Tissue Antigens |
| Abstract |
The MHC-DRB1 gene is known to display the most extensive allelic polymorphisms among MHC class II genes. We attempted the selective identification of chimpanzee (Pan troglodytes) DRB1 (Patr-DRB1) alleles using the polymerase chain reaction (PCR) technique in three steps: first, we performed Patr-DRB1*02 lineage-specific 8-kb PCR for *02 lineage detection in each chimpanzee; second, we performed 620-bp PCR for amplification of full-length exon 2; and finally, we carried out an insert check using the pattern of microsatellite repeat length variability. In the genomic DNA of 23 chimpanzees, nine Patr-DRB1 alleles containing two new alleles were detected. Our approach provides a relatively effective method of identifying Patr-DRB1 alleles in individual chimpanzees and should also contribute to our understanding of the features of MHC molecules in non-human primates. |
| Volume | 67(2) |
| Pages | 134-42 |
| Published | 2006-2-1 |
| DOI | 10.1111/j.1399-0039.2006.00539.x |
| PII | TAN539 |
| PMID | 16441484 |
| MeSH | Alleles* Amino Acid Sequence Animals Base Sequence Cloning, Molecular Genes, MHC Class II Genetic Variation* HLA-DR Antigens / genetics* HLA-DRB1 Chains Microsatellite Repeats Molecular Sequence Data Pan troglodytes / genetics* Pan troglodytes / immunology Polymorphism, Genetic* Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Species Specificity |
| IF | 1.348 |
| Times Cited | 5 |
| WOS Category | PATHOLOGY IMMUNOLOGY CELL BIOLOGY |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
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