| RRC ID |
19698
|
| Author |
Sanei M, Pickering R, Kumke K, Nasuda S, Houben A.
|
| Title |
Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Uniparental chromosome elimination occurs in several interspecific hybrids of plants. We studied the mechanism underlying selective elimination of the paternal chromosomes during the early development of Hordeum vulgare × Hordeum bulbosum embryos. The following conclusions regarding the role of the centromere-specific histone H3 variant (CENH3) in the process of chromosome elimination were drawn: (i) centromere inactivity of H. bulbosum chromosomes triggers the mitosis-dependent process of uniparental chromosome elimination in unstable H. vulgare × H. bulbosum hybrids; (ii) centromeric loss of CENH3 protein rather than uniparental silencing of CENH3 genes causes centromere inactivity; (iii) in stable species combinations, cross-species incorporation of CENH3 occurs despite centromere-sequence differences, and not all CENH3 variants get incorporated into centromeres if multiple CENH3s are present in species combinations; and (iv) diploid barley species encode two CENH3 variants, the proteins of which are intermingled within centromeres throughout mitosis and meiosis.
|
| Volume |
108(33)
|
| Pages |
E498-505
|
| Published |
2011-8-16
|
| DOI |
10.1073/pnas.1103190108
|
| PII |
1103190108
|
| PMID |
21746892
|
| PMC |
PMC3158150
|
| MeSH |
Centromere / chemistry*
Chimera
Chromosomes, Plant*
Histones / metabolism*
Hordeum / genetics*
Meiosis
Mitosis
Molecular Sequence Data
Plant Proteins / metabolism
Uniparental Disomy
|
| IF |
9.412
|
| Times Cited |
139
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Wheat |
|
