RRC ID 19823
Author Iseki M, Matsunaga S, Murakami A, Ohno K, Shiga K, Yoshida K, Sugai M, Takahashi T, Hori T, Watanabe M.
Title A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis.
Journal Nature
Abstract Blue light regulates processes such as the development of plants and fungi and the behaviour of microbes. Two types of blue-light receptor flavoprotein have been identified: cryptochromes, which have partial similarity to photolyases, and phototropins, which are photoregulated protein kinases. The former have also been found in animals with evidence of essential roles in circadian rhythms. Euglena gracilis, a unicellular flagellate, abruptly changes its swimming direction after a sudden increase or decrease in incident blue light intensity, that is, step-up or step-down photophobic responses, resulting in photoavoidance or photoaccumulation, respectively. Although these photobehaviours of Euglena have been studied for a century, the photoreceptor molecules mediating them have remained unknown. Here we report the discovery and biochemical characterization of a new type of blue-light receptor flavoprotein, photoactivated adenylyl cyclase, in the photoreceptor organelle of Euglena gracilis, with molecular genetic evidence that it mediates the step-up photophobic response.
Volume 415(6875)
Pages 1047-51
Published 2002-2-28
DOI 10.1038/4151047a
PII 4151047a
PMID 11875575
MeSH Adenylyl Cyclases / genetics Adenylyl Cyclases / metabolism Adenylyl Cyclases / radiation effects* Amino Acid Sequence Animals Enzyme Activation / radiation effects Euglena gracilis Flavoproteins / genetics Flavoproteins / metabolism Flavoproteins / radiation effects Gene Expression Light Molecular Sequence Data Organelles / metabolism Photoreceptor Cells, Invertebrate / enzymology* Photoreceptor Cells, Invertebrate / radiation effects Sequence Alignment
IF 41.577
Times Cited 257
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Algae NIES-48